The PsCZF1 gene encoding a C2H2 zinc finger protein is required for growth, development and pathogenesis in Phytophthora sojae

Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
Microbial Pathogenesis (Impact Factor: 1.79). 06/2009; 47(2):78-86. DOI: 10.1016/j.micpath.2009.04.013
Source: PubMed


The C(2)H(2) zinc finger proteins form one of the largest families of transcriptional regulators in eukaryotes. We identified a Phytophthora sojae C(2)H(2) zinc finger (PsCZF1), that is highly conserved in sequenced oomycete pathogens. In transformants of P. sojae containing the PsCZF1 promoter fused to the beta-glucuronidase (GUS) reporter gene, GUS activity was highly induced in the P. sojae oospore stage and upregulated after infection. To elucidate the function of PsCZF1, its expression was silenced by introducing anti-sense constructs into P sojae. PsCZF1-silenced transformants did not exhibit altered cell size or morphology of sporangia and hyphae; however, hyphal growth rate was reduced by around 50% in the mutants. PsCZF1-deficient mutants were also impaired in production of oospores, swimming zoospores and germinating cysts, indicating that the gene is involved in various stages of the life cycle. Furthermore, we found that PsCZF1-deficient mutants lost virulence on host soybean cultivars. Our results suggest that this oomycete-specific C(2)H(2)-type zinc finger protein plays an important role in growth, development, and pathogenesis; therefore, PsCZF1 might be an attractive oomycete-specific target for chemical fungicide screening.

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Available from: Xiao-Ren Chen, Sep 03, 2015
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    • "Among these distinct types of zinc finger proteins, the C2H2‐type zinc finger proteins are one of the largest transcriptional factor families in plants, with 176 members in Arabidopsis identified through in silico analysis (Englbrecht et al. 2004; Ciftci‐Yilmaz and Mittler 2008). To date, C2H2 zinc finger proteins have been found to participate in extraordinarily diverse signal transduction pathways and developmental processes, including flower development (Bowman et al. 1992; Sakai et al. 1995; Yun et al. 2002; Xiao et al. 2009), seed and seeding development (Luo et al. 1999; Prigge and Wagner 2001), trichome and root hair formation (Gan et al. 2006, 2007a; Zhou et al. 2011, 2013; An et al. 2012b), pathogen defense (McGrath et al. 2005; Kazan 2006; Wang et al. 2009), and stress responses (Kazan 2006; Sun et al. 2010; Tian et al. 2010; Kiełbowicz‐Matuk 2012; Zhang et al. 2012). "
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    ABSTRACT: Cell fate determination is a basic developmental process during the growth of multicellular organisms. Trichomes and root hairs of Arabidopsis are both readily accessible structures originating from the epidermal cells of the aerial tissues and roots respectively, and they serve as excellent models for understanding the molecular mechanisms controlling cell fate determination and cell morphogenesis. The regulation of trichome and root hair formation is a complex program that consists of the integration of hormonal signals with a large number of transcriptional factors, including MYB and bHLH transcriptional factors. Studies during recent years have uncovered an important role of C2H2 type zinc finger proteins in the regulation of epidermal cell fate determination. Here in this mini-review we briefly summarize the involvement of C2H2 zinc finger proteins in the control of trichome and root hair formation in Arabidopsis.
    Journal of Integrative Plant Biology 05/2014; 56(12). DOI:10.1111/jipb.12221 · 3.34 Impact Factor
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    • "For quantifying oospore production 1 cm 2 was cut from a 7-day old mycelial culture on LBA medium. This plug was comminuted with a homogenizer (Fluko) and the suspension was examined by microscopy (Wang et al., 2009). "
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    ABSTRACT: 科研費報告書収録論文(課題番号:09680388・基盤研究(C)(2)・H9~H10/研究代表者:根元, 義章/情報フィルタリングを用いた大規模情報ネットワークのリアルタイム障害検出方式)
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