Matrine induces apoptosis in gastric carcinoma cells via alteration of Fas/FasL and activation of caspase-3
ABSTRACT Matrine, an alkaloid purified from the chinese herb Sophora flavescens Ait, is well known to possess activities including anti-inflammation, anti-fibrotic and anticancer. In this study, the mechanism of matrine inducing the apoptosis of gastric carcinoma cells was investigated.
Proliferation of SGC-7901 cells was examined by MTT assay. Cellular morphology was observed under transmission electron microscope. Flow cytometry (FCM) was used to observe the apoptosis of SGC-7901 cells by staining with annexinV-FITC/PI. The expression levels of Fas/FasL in SGC-7901 cells were monitored by FCM analysis using an indirect immunofluorescence method. Activity of caspase-3 enzyme was measured by spectrofluorometry.
MTT assay showed that matrine inhibited SGC-7901 cells proliferation in a dose-dependent and time-dependent manner. Apoptosis induction was demonstrated by morphological changes under electron microscope and FCM analysis. Fluorescence intensity levels of Fas and FasL were found to be equally up-regulated after matrine treatment, which were both correlated with apoptosis rate. The activity of caspase-3 enzyme increased in matrine groups, positively correlated with apoptosis rate.
Matrine could inhibit cell proliferation and induce apoptosis of SGC-7901 cells in vitro. The apoptosis induction appears to proceed by up-regulating Fas/FasL expression and activating caspase-3 enzyme.
SourceAvailable from: Ning Wang[Show abstract] [Hide abstract]
ABSTRACT: Chinese medicines have long history in treating cancer. With the growing scientific evidence of biomedical researches and clinical trials in cancer therapy, they are increasingly accepted as a complementary and alternative treatment. One of the mechanisms is to induce cancer cell death. Aim. To comprehensively review the publications concerning cancer cell death induced by Chinese medicines in recent years and provide insights on anticancer drug discovery from Chinese medicines. Materials and Methods. Chinese medicines (including Chinese medicinal herbs, animal parts, and minerals) were used in the study. The key words including "cancer", "cell death", "apoptosis", "autophagy," "necrosis," and "Chinese medicine" were used in retrieval of related information from PubMed and other databases. Results. The cell death induced by Chinese medicines is described as apoptotic, autophagic, or necrotic cell death and other types with an emphasis on their mechanisms of anticancer action. The relationship among different types of cell death induced by Chinese medicines is critically reviewed and discussed. Conclusions. This review summarizes that CMs treatment could induce multiple pathways leading to cancer cell death, in which apoptosis is the dominant type. To apply these preclinical researches to clinic application will be a key issue in the future.BioMed Research International 01/2014; 2014:530342. DOI:10.1155/2014/530342 · 2.71 Impact Factor
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ABSTRACT: The objective was to analyze the relation between gastric cancer susceptibility and gene polymorphism, providing a reference for epidemiologic research of gastric cancer etiology. Two hundred and eighty gastric cancer cases were selected, and 280 healthy cases with the same gender, age (±5), and residence place were selected as control group, with proportion of 1:1. Tag single nucleotide polymorphism was used for screening polymorphism of caspase3, which was combined with logistic regression model and multi-point joint analysis to analyze relation between different genotypes and gastric susceptibility. In analysis of gene polymorphism of caspase3 intrinsic apoptotic pathway and gastric cancer susceptibility, polymorphism of CASP3 rs4647693, CASP3 rs12108497 and CASP3 rs4647610 increased gastric cancer risk (rs4647693: ORGA 1.61, 95 % CI 1.06-2.28; rs12108497: ORTC 1.55, 95 % CI 1.09-2.18; ORCC 2.45, 95 % CI 1.08-4.16; rs4647610: ORAG 1.71, 95 % CI 1.14-2.31; ORGG 1.60, 95 % CI 1.23-2.34). Gastric cancer risk of haplotype AGGC carrier was significantly higher than that of haplotype GGAT as control (OR 1.44, 95 % CI 1.07-2.19). Gene polymorphism and haplotype of caspase3 can increase gastric cancer risk. However, it still needs to be verified by a large-sample and multicenter epidemiologic research.Cell Biochemistry and Biophysics 07/2014; 70(3). DOI:10.1007/s12013-014-0108-0 · 2.38 Impact Factor