Article

The osteoprotective effect of Radix Dipsaci extract in ovariectomized rats

Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China.
Journal of ethnopharmacology (Impact Factor: 2.94). 06/2009; 123(1):74-81. DOI: 10.1016/j.jep.2009.02.025
Source: PubMed

ABSTRACT The objective of the present study was to systematically evaluate the effects of Radix Dipsaci extract (RDE) on postmenopausal osteoporosis.
OVX or sham operations were performed on sixty 3-month-old virgin Sprague-Dawley rats that were divided into six groups: sham control group (sham, n=10); OVX control group (OVX, n=10); 17beta-estradiol treatment group (E2, n=10); three Radix Dipsaci extract treatment groups RDE100 (n=10), RDE300 (n=10) and RDE500 (n=10). The treatment began 4 weeks after the surgery and lasted for 16 weeks. Bone mass, bone turnover and strength were analyzed by DEXA, biochemical markers and three-point bending test. The trabecular bone microarchitecture was evaluated by MicroCT.
16 weeks treatment of RDE slowed down the body weight gain and prevented the loss of bone mass induced by the OVX. The prevention effect on bone loss was due to altering the rate of bone remodeling, which could be inferred from the decreased level of bone turnover markers, such as serum ALP, OC and urinary DPD. The changes of urinary calcium and phosphorus excretion provided the same evidence. The treatment could also enhance the bone strength and prevent the deterioration of trabecular microarchitecture.
Our study provides evidence that Radix Dipsaci extract will have potential to be used for treatment of postmenopausal osteoporosis.

0 Followers
 · 
166 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: Six new compounds, including four new lignans, dipsalignan A (1), B-D (3-5), and two new bis-iridoid glycoside dimmers, dipsanosides M (7) and N (8), together with two known compounds (2) and (6), have been isolated from the roots of Dipsacus asper Wall. Their structures were established on the basis of spectroscopic data (MS, 1D, 2D NMR, and CD) and chemical methods. All the isolated compounds were tested against human immunodeficiency virus-1 (HIV-1) integrase inhibition activities, and only compounds 1, 2, 7, and 8 displayed weak activities.
    Molecules 02/2015; 20(2):2165-2175. DOI:10.3390/molecules20022165 · 2.10 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Falcaria vulgaris is an important medicinal plant belonging to the family Apiaceae. The leaf of explants of this plant was cultured for callus induction and plant regeneration. The explants of this plant were cultured onto Murashig and Skoog (MS) medium supplemented with different concentrations of (alpha)-naphtalene acetic acid (NAA), 2,4-dichlorophenoxy acetic acid (2,4-D), thidiazuron (TDZ), alone and in combination with 6- benzyladenine (BA) for callus induction. The highest callus was induced in medium containing (0.5 and 1.0 mg L-1) 2,4-D in combination with BA. These callus and leaf segments were transferred to MS medium supplemented with different combination of NAA and BA for indirect and direct regeneration, respectively. The medium containing (1.0 mg L-1) NAA in combination with (0.5 and 1.0 mg L-1) BA showed the highest number of shoot and root formation in plant regeneration through the callus. In direct regeneration, NAA with 1.0 mg L-1 concentration was observed to be more potent than with concentration of 0.5 mg L-1 and showed highest root regeneration frequency (15.7%). In vitro raised plantlets were acclimatized onto natural condition with 90% survival. These results provide a basis for future studies on genetic improvement and could be applied to production of secondary metabolites through cell culture in Falcaria vulgaris.
    Journal of medicinal plant research 01/2012; 6(18):3407-3414. · 0.88 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Traditional Chinese Medicine (TCM) Fufang or formula Xianlinggubao (XLGB) is a prescribed TCM drug in China registered for prevention and treatment of osteoporosis. Fufang in TCM is comprised of a group of herbal compounds contributing in group to the treatment efficacy. The present study aims to identify the bioactive fraction(s) in XLGB extract that account(s) dominantly for its osteogenic effects. The extract of XLGB formula was separated into three fractions using chromatography, i.e., XLGB-A, XLGB-B and XLGB-C. They were administrated to 4-month old ovariectomized (OVX) mice for 6 weeks to determine which bioactive fraction(s) were more effective for preventing OVX-induced bone loss evaluated by microCT, biomechanical testing and biochemical markers. The main peaks of the key fraction were identified using reference compounds isolated from the fraction. In addition, the effects of the composite compounds in XLGB-B on osteoblasts' proliferation and mineralization were evaluated in UMR 106 cells. XLGB-B with a yield of 13.0% from herbal Fufang XLGB was identified as the most potential one among the three fractions for prevention of OVX-induced bone loss confirmed with bone mass, bone microarchitecture, bone strength and bone turnover markers. Nine compounds in HPLC fingerprint were identified in the XLGB-B fraction, including phenylpropanoids from Herba Epimedii, terpenes from Radix Dipsaci and coumarins from Fructus Psoraleae. In addition, the identified compounds effectively promoted proliferation and/or mineralization of osteoblast-like UMR 106 cells in vitro. XLGB-B with defined phytochemical structures was screened as the key fraction that demonstrated preventive effects on OVX-induced bone loss in mice. The present study laid down a foundation towards a new generation of herbal Fufang characterized with "less herbal materials for achieving equal treatment efficacy" in development strategy of TCM for prevention of OVX-induced osteoporosis.
    PLoS ONE 02/2015; 10(2):e0118184. DOI:10.1371/journal.pone.0118184 · 3.53 Impact Factor