TESA-blot for the diagnosis of Chagas disease in dogs from co-endemic regions for Trypanosoma cruzi, Trypanosoma evansi and Leishmania chagasi

Universidade de São Paulo - Instituto de Medicina Tropical de São Paulo, SP, Brazil.
Acta tropica (Impact Factor: 2.27). 08/2009; 111(1):15-20. DOI: 10.1016/j.actatropica.2009.01.006
Source: PubMed


We standardized serodiagnosis of dogs infected with Trypanosoma cruzi using TESA (trypomastigote excreted-secreted antigen)-blot developed for human Chagas disease. TESA-blot showed 100% sensitivity and specificity. In contrast, ELISA using TESA (TESA-ELISA) or epimastigotes (epi-ELISA) as antigen yielded 100% sensitivity but specificity of 94.1% and 49.4%, respectively. When used in field studies in an endemic region for Chagas disease, visceral leishmaniasis and Trypanosoma evansi (Mato Grosso do Sul state, Central Brazil), positivities were 9.3% for TESA-blot, 10.7% for TESA-ELISA and 32% for epi-ELISA. Dogs from a non-endemic region for these infections (Rondonia state, western Amazonia) where T. cruzi is enzootic showed positivity of 4.5% for TESA-blot and epi-ELISA and 6.8% for TESA-ELISA. Sera from urban dogs from Santos, São Paulo, where these diseases are absent, yielded negative results. TESA-blot was the only method that distinguished dogs infected with T. cruzi from those infected with Leishmania chagasi and/or Trypanosoma evansi.

Download full-text


Available from: Mary Marcondes,
  • Source
    • "In dog, surra usually follows an acute course of infection (Ravindran et al., 2008). Although in dogs, the disease is usually sporadic, outbreaks of canine trypanosomiasis have been reported from India, Brazil, Iran and South America (Herrera et al., 2004; Ian et al., 2004; Morteza et al., 2007; Umezawa et al., 2009; Eloy and Lucheis, 2009). Of the "
    [Show abstract] [Hide abstract]
    ABSTRACT: The present report describes a case of Trypanosoma evansi infection (surra) in a 5 months old male bulldog pup weighing 13 kg. Clinical signs included a moderate fever (39.6 o C), pulse (140 beats/min) and an accelerated respiration rate (45/min; normal 10–30/min), anemia, watery eyes and dehydration. Microscopic examination of wet blood films revealed an extremely large number of trypo-mastigotes and thin blood smears stained with Dip Quick™ stain revealed a large numbers of trypanosomes with a characteristic flagellum, kinetoplast and undulating membrane. The pup was treated with 4 mg Cymelarsan® (Rhone Meriux, France) powder dissolved in 3 ml distilled water and administered by deep intramuscular injection once and oral administration of 100 mg manganese chloride dissolved in 10 ml water daily for 5 days. Wet blood films and Dip Quick ™ stained blood smears were negative for trypanosomes on day 3, 7, and 14 of post treatment and all vital parameters returned to normal on day–4 post–medication. MS (2014). Use of Cymelarsan ® and Manganese Chloride for Treatment of the Canine Trypanosomiasis (Surra): A Research report. Adv. Anim. Vet. Sci. 2 (2): 104 – 105. Surra is a protozoan disease caused by Trypanosoma evansi that affects a wide variety of mammalian hosts. Several reports have described the occurrence of surra in adult dogs (Rashid et al., 2008; Eloy and Lucheis, 2009; Defontis et al., 2012). However, as far as could be ascertained, documentation of treatment of this disease in pups doesn't exist. The present report describes surra in a 5–month old bulldog male pup and subsequent treatment.
    Advances in Animal and Veterinary Sciences 07/2014; 2(2):104-105.
    • "The OligoC-TesT has never been evaluated in dogs and this study indicates that the test is not compatible with blood samples from dogs and needs further optimization. Molecular tests like OligoC-TesT are potential confirmatory tests as they are more specific than serological tests,[15] which are subjected to cross reaction with other pathogens.[51920] The poor performance of the OligoC-TesT made difficult comparing both tests. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Introduction: Chagas disease is a zoonotic disease caused by Trypanosoma cruzi and dogs are one of the main domestic reservoirs. Materials and Methods: One molecular (OligoC-TesT, Coris Bioconcept) and one serological (T. cruzi-Detect, Inbios) rapid tests were evaluated as infection markers for T. cruzi in 102 dogs living in eight villages endemic for Chagas in Costa Rica. Results: T. cruzi-Detect performed well as screening tool with 23.3% positive samples. The large number of invalid results (66.7%) observed in samples tested with OligoC-TesT precluded assessing the use of this new method as epidemiological tool to detect T. cruzi infection in dogs.
    07/2014; 4(2):111-4. DOI:10.4103/2229-5070.138539
  • Source
    • "Dogs naturally infected with T. cruzi or T. evansi showed different positivities with studied parasites with exception of T. cruzi antigen that resulted in positivity of 100% (Table 1Tryps). These data were not surprising because previously it had been demonstrated that sera from hosts infected with T. evansi react with T. cruzi parasites (Umezawa et al., 2009), and the reverse scenario also occurs (Desquesnes et al., 2007). These results indicated that L. (L.) chagasi and the parasites C. luciliae, C. fasciculata and L. seymouri share similar antigenic molecules, probably in accordance with their close phylogenetic relationship. "
    [Show abstract] [Hide abstract]
    ABSTRACT: We investigated whether ELISA using crude antigens from insect and plant trypanosomatids, which are non-pathogenic and easily cultivated in large scale, has the same positivity data as Leishmania (Leishmania) chagasi, the etiological agent of human visceral leishmaniasis (VL) or canine leishmaniasis (CanL), or as T. cruzi, the etiological agent of Chagas disease (CD). The antigens from Crithidia fasciculata, Crithidia luciliae, and Leptomonas seymouri showed 100% cross-reactivity with VL and CanL samples, with no statistically titers differences from L. (L.) chagasi, however, 34% (17/50) of VL samples revealed higher titers using the insect trypanosomatids than the homologous antigen. On the other hand, antigens from Strigomonas culicis, Angomonas deanei, and Phytomonas serpens showed low cross-reactivity with VL and CanL samples. The sera from patients with American tegumentary leishmaniasis showed low levels of cross-reactivity with all trypanosomatids investigated, even with L. (L) chagasi, without titers dissimilarity among them. These parasites were also worthless as antigen source for detection of CD cases, which required homologous antigens to reach 100% positivity. This study showed, by ELISA, that crude extract of Crithidia and Leptomonas have epitopes similar to L. (L.) chagasi, which supports the idea of using them as antigens source for the serodiagnosis of visceral leishmaniasis.
    Acta tropica 11/2013; 131(1). DOI:10.1016/j.actatropica.2013.11.010 · 2.27 Impact Factor
Show more