Role of IL-10 Deficiency in Pneumonia Induced by Corynebacterium kutscheri in Mice

College of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea.
Journal of Microbiology and Biotechnology (Impact Factor: 1.53). 05/2009; 19(4):424-30. DOI: 10.4014/jmb.0807.436
Source: PubMed


IL-10 is an important anti-inflammatory cytokine that can inhibit the production of many pro-inflammatory cytokines. Both human and animal studies have shown that pro-inflammatory cytokines play an important role in pneumonia and other inflammatory lung diseases. In the present study, IL-10 knockout (KO) and wild-type mice were infected with Corynebacterium kutscheri to determine whether the severity of pathogenesis and whether protective immunity could be altered in the absence of IL- 10. The survival rate was significantly lower in IL-10 KO mice than wild-type mice. The number of neutrophils in bronchoalveolar lavage fluid and blood were found to be higher in IL-10 KO mice than wild-type mice. IL-10 KO mice showed greater neutrophil infiltration, excessive inflammation, and weight-loss compared with wild-type mice. Furthermore, upregulation of IFN-gamma in bronchoalveolar lavage fluid, and upregulation of MIP-1alpha and IP-10 mRNA in the lungs of IL-10 KO mice compared with wild-type mice after C. kutscheri infection were observed. These results suggest that IL-10 plays an important role in the anti-inflammatory properties against C. kutscheri infection, and that lack of IL-10 leads to a more severe pulmonary inflammatory response. This increased susceptibility to C. kutscheri pneumonia is at least in part caused by IL-10 deficiency and severe recruitment of neutrophils.

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    • "opponent effect on host–pathogen interaction. On one hand, IL-10 may lead to dysfunctional immune protection, thus providing an opportunity for immune evasion by the microbes (McGuirk and Mills, 2002; Ocana-Morgner et al., 2003; Jeong et al., 2009). On the other hand, IL-10 production may be beneficial to the host, given the fact that IL-10 is essential in regulating immune responses and thus preventing an excessive inflammatory response that may be detrimental (Akbari et al., 2001; Higgins et al., 2003). "
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    Cell Biology International 03/2011; 35(8):793-8. DOI:10.1042/CBI20100463 · 1.93 Impact Factor
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    • "Although neutrophils are usually considered important participants to combat extracellular bacterial infections, they are also prominent components of inflammatory responses induced by numerous viral infections (Andrews et al., 1999; Sakai et al., 2000; Wang and Forsyth, 2000). However, in absence of the anti-inflammatory milieu provided by IL-10 to suppress immune responses, their increased recruitment could contribute to pathology (Jeong et al., 2009). "
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