Antifungal activity of black tea polyphenols (catechins and theaflavins) against Candida species.
ABSTRACT The polyphenols catechins and theaflavins in black tea have been shown to possess many medicinal properties, including anticancer activity and some antifungal characteristics, but there have been few studies of their anti-Candida activity. In this paper we report the results of our study of the anti-Candida activity of tea polyphenols.
The effects of 4 different concentrations of catechins and theaflavins were evaluated on 5 isolates each of 5 Candida species employing an agar diffusion growth inhibition assay. The minimum inhibitory concentration (MIC) of the polyphenols against C. albicans was determined. The post-antifungal effect (PAFE) of the polyphenols for C. albicans was investigated. C. albicans cells exposed to polyphenols were studied using a scanning electron microscope (SEM).
Both polyphenols showed anti-Candida activity against all tested Candida species and demonstrated a MIC of 6.25 mg/ml for C. albicans. C. glabrata was found to be the most sensitive species followed by C. parapsilosis, C. albicans, C. krusei and C. tropicalis (p < 0.05 for all). Significant intraspecies variations in sensitivity were noted among C. parapsilosis and C. tropicalis (p < 0.001) for both polyphenols. Theaflavins displayed standard PAFE while catechins showed a paradoxical PAFE with all isolates of C. albicans. SEM revealed considerable cell wall damage of C. albicans cells exposed to the polyphenols.
The study reveals for the first time the anti-Candida properties of black tea polyphenols that may find therapeutic applications in future.
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ABSTRACT: The tea polyphenols have been shown to possess many medicinal properties including antifungal activity, but there have been few studies regarding antifungal activity. The antifungal activity of tea polyphenols was evaluated on Candida albicans ATCC 90028 and a clinical isolate of Cryptococcus neoformans employing the disc diffusion assay. The minimum inhibitory concentration (MIC) of the tea polyphenols against C. albicans ATCC 90028 and a clinical isolate of C. neoformans was determined. Tea polyphenols showed antifungal activity against C. albicans ATCC 90028 and a clinical isolate of C. neoformans and both demonstrated an MIC of 1 mg/ml after 24 h. Both fungus were found to be sensitive to tea all tea extracts (p<0.05). The inhibition zone diameters significantly (p<0.05) and positively correlated to the catechins (EGCG and EGC), total TFs and total TRs. The study reveals the antifungal properties of green, white and black tea products from Kenyan germplasm that may find therapeutic applications in future. Key words: Tea polyphenols, Candida albicans, Cryptococcus neoformans.African journal of agricultural research 05/2013; · 0.08 Impact Factor
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ABSTRACT: The prevalence of antibiotic resistance has resulted in the need for new approaches to be developed to combat the previously easily treatable infections. This work aims to evaluate the antifungal and antioxidant effects of the chitosan, as a new alternative or complementary anti-fungal drug, alone or in combination with amphotericin B against a pathogenic Candida albicans in mice. Eighty neutropenic infected mice were randomly assigned into four main groups (20 mice/group). The 1st group was treated with saline, neutropenic infected (NI group) (IPC group, invasive pulmonary candidiasis), the 2nd group was treated with chitosan (ED50) (CE group), the 3rd group was treated with amphotericin B (150 mg/kg) (AMB group) and the 4th group was treated with chitosan plus amphotericin B (CE + AMB group). Treatment was started at 24 h after fungal inoculation and was administered for 3 consecutive days. All the previous treatments demonstrated notable growth inhibition against a C. albicans isolate as indicated by measuring the mean diameter of the inhibition zone. Compared with IPC group, CE, AMB, and AMB + CE-treated animals had 73%, 87%, and 90% reduction in fungal burden, respectively. Furthermore, treatment with CE and/or AMB for 24 and 72 h significantly decreased MDA, SOD, CAT and NO levels and increased GSH and in the lung tissues as compared with the infected untreated group. In conclusion, CE treatment, with the combination of antifungal therapy, can alleviate oxidative stress and lung injury associated with IPC in neutropenic mice.The Journal of Basic & Applied Zoology. 01/2015; 105.
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ABSTRACT: Nigella sativa L. commonly called black seed belongs to the family Ranunculaceae is known for its indigenous medicinal uses since immemorial time. The present study was carried out in order to characterize the Nigella seed oil cultivated in Morocco for its fatty acid and sterol composition using gas chromatography. The results indicated the presence of linoleic acid (58.5%) and oleic acid (23.7%) as unsaturated fatty acids and palmitic acid (13.1%) as saturated fatty acid while β-sitosterol and stigmasterol were among the major components, together constituting about 69 % of total sterols. Nigella seed oil has a strong antifungal activity compared to the conventional fungicides. Our findings demonstrate that Nigella seed oil has an antifungal activity that might be a natural potential source of antifungal used in food, in cosmetics and in pharmaceuticals products.International Journal of Pharmaceutical Science Invention. 11/2014; 3(11):2319-671809.