Article

Audio frequency in vivo optical coherence elastography.

Optical+Biomedical Engineering Laboratory, School of Electrical, Electronic & Computer Engineering, The University of Western Australia, Crawley, Western Australia, Australia.
Physics in Medicine and Biology (Impact Factor: 2.92). 06/2009; 54(10):3129-39. DOI: 10.1088/0031-9155/54/10/011
Source: PubMed

ABSTRACT We present a new approach to optical coherence elastography (OCE), which probes the local elastic properties of tissue by using optical coherence tomography to measure the effect of an applied stimulus in the audio frequency range. We describe the approach, based on analysis of the Bessel frequency spectrum of the interferometric signal detected from scatterers undergoing periodic motion in response to an applied stimulus. We present quantitative results of sub-micron excitation at 820 Hz in a layered phantom and the first such measurements in human skin in vivo.

Download full-text

Full-text

Available from: Brendan F Kennedy, Jul 06, 2015
0 Followers
 · 
129 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Dynamic optical coherence elastography, an emerging optical technique to measure material mechanical properties using the non-invasive imaging modality of optical coherence tomography is introduced. Dynamic mechanical excitations were applied to the samples while a spectral domain optical coherence tomography system was used for detection. Based on a simple mechanical model, material mechanical properties such as Young's moduli can be extracted from detected phase-resolved signals. Biological tissues and their biomechanical properties are currently the main objects for this technique due to its micron-scale resolution and relatively deep penetration. Quantitative results were achieved by this technique on tissue phantoms and rat tumor tissues. Different excitation approaches and applications for dynamic optical coherence elastography are also discussed.
    Communications and Photonics Conference and Exhibition (ACP), 2009 Asia; 12/2009
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Biomechanical properties are important for living tissues and cells. They are indicators of functional changes and pathological variations in the micro-structure, such as during tumor development. The topic of this thesis falls at the intersection of biomechanics and optical imaging, and focuses on optical elastography, an optical sensing and imaging technique used to measure biomechanical properties at the tissue and cell levels. Optical coherence elastography, multiphoton elastography, and magnetomotive microscopy are developed, demonstrated, optimized, and applied at the tissue level to ex vivo breast cancer and in vivo skin, and at the cellular level to mouse macrophages in culture. Driven by scientific needs to engineer new quantitative methods that utilize the high micro-scale resolution achievable with optics, results of biomechanical properties were obtained from the biological samples. The results suggest potential diagnostic and therapeutic clinical applications. Results from these studies also help our understanding of the relationship between biomechanical variations and tissue/cell functional changes in biological systems. Therefore, the engineering of imaging techniques is employed to investigate biomechanics, as well as the feasibility for using these techniques to solve more scientific and clinical questions.
  • Source