Article

A nanostructured cerium oxide film-based immunosensor for mycotoxin detection.

National Physical Laboratory, New Delhi 110012, India.
Nanotechnology (impact factor: 3.98). 03/2009; 20(5):055105. DOI:10.1088/0957-4484/20/5/055105 pp.055105
Source: PubMed

ABSTRACT Rabbit-immunoglobulin antibodies (r-IgGs) and bovine serum albumin (BSA) have been immobilized onto sol-gel-derived nanostructured cerium oxide (nanoCeO(2)) film fabricated onto an indium-tin-oxide (ITO) coated glass plate to detect ochratoxin-A (OTA). Broad reflection planes obtained in x-ray diffraction (XRD) patterns reveal the formation of CeO(2) nanostructures. Electrochemical studies reveal that nanoCeO(2) particles provide an increased electroactive surface area for loading of r-IgGs with desired orientation, resulting in enhanced electron communication between r-IgGs and electrode. BSA/r-IgGs/nano CeO(2)/ITO immunoelectrode exhibits improved characteristics such as linear range (0.5-6 ng dl(-1)), low detection limit (0.25 ng dl(-1)), fast response time (30 s) and high sensitivity (1.27 microA ng(-1) dl(-1) cm(-2)). The high value of the association constant (K(a), 0.9 x 10(11) l mol(-1)) indicates the high affinity of the BSA/r-IgGs/nanoCeO(2)/ITO immunoelectrode to OTA.

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    Article: Prospects of nanotechnology in clinical immunodiagnostics.
    Anees A Ansari, Mansour Alhoshan, Mohamad S Alsalhi, Abdullah S Aldwayyan
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    Sensors 01/2010; 10(7):6535-81. · 1.74 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

affinity
 
bovine serum albumin
 
BSA/r-IgGs/nano CeO(2)/ITO immunoelectrode exhibits
 
BSA/r-IgGs/nanoCeO(2)/ITO immunoelectrode
 
characteristics
 
desired orientation
 
Electrochemical studies
 
electron communication
 
fast response time
 
glass plate
 
increased electroactive surface area
 
low detection limit
 
Rabbit-immunoglobulin antibodies
 
x-ray diffraction