A nanostructured cerium oxide film-based immunosensor for mycotoxin detection

National Physical Laboratory, New Delhi 110012, India.
Nanotechnology (Impact Factor: 3.82). 03/2009; 20(5):055105. DOI: 10.1088/0957-4484/20/5/055105
Source: PubMed


Rabbit-immunoglobulin antibodies (r-IgGs) and bovine serum albumin (BSA) have been immobilized onto sol-gel-derived nanostructured cerium oxide (nanoCeO(2)) film fabricated onto an indium-tin-oxide (ITO) coated glass plate to detect ochratoxin-A (OTA). Broad reflection planes obtained in x-ray diffraction (XRD) patterns reveal the formation of CeO(2) nanostructures. Electrochemical studies reveal that nanoCeO(2) particles provide an increased electroactive surface area for loading of r-IgGs with desired orientation, resulting in enhanced electron communication between r-IgGs and electrode. BSA/r-IgGs/nano CeO(2)/ITO immunoelectrode exhibits improved characteristics such as linear range (0.5-6 ng dl(-1)), low detection limit (0.25 ng dl(-1)), fast response time (30 s) and high sensitivity (1.27 microA ng(-1) dl(-1) cm(-2)). The high value of the association constant (K(a), 0.9 x 10(11) l mol(-1)) indicates the high affinity of the BSA/r-IgGs/nanoCeO(2)/ITO immunoelectrode to OTA.

Download full-text


Available from: Ajeet Kaushik,
56 Reads
  • Source
    • "Ansari et al. utilized Nano-ZnO film deposited on an indium-tin-oxide (ITO) with immobilized rabbit-immunoglobulin and bovine serum albumin to reach a detection limit of 0.006 nM/dm of the toxin. Similarly, Kaushik et al. used nanostructured cerium oxide to achieve a detection limit of 0.25ng/dL of ochratoxin (Kaushik et al., 2009a "
    [Show abstract] [Hide abstract]
    ABSTRACT: Abstract The post-genomics era has brought about new Omics biotechnologies, such as proteomics and metabolomics, as well as their novel applications to personal genomics and the quantified self. These advances are now also catalyzing other and newer post-genomics innovations, leading to convergences between Omics and nanotechnology. In this work, we systematically contextualize and exemplify an emerging strand of post-genomics life sciences, namely, nanoproteomics and its applications in health and integrative biological systems. Nanotechnology has been utilized as a complementary component to revolutionize proteomics through different kinds of nanotechnology applications, including nanoporous structures, functionalized nanoparticles, quantum dots, and polymeric nanostructures. Those applications, though still in their infancy, have led to several highly sensitive diagnostics and new methods of drug delivery and targeted therapy for clinical use. The present article differs from previous analyses of nanoproteomics in that it offers an in-depth and comparative evaluation of the attendant biotechnology portfolio and their applications as seen through the lens of post-genomics life sciences and biomedicine. These include: (1) immunosensors for inflammatory, pathogenic, and autoimmune markers for infectious and autoimmune diseases, (2) amplified immunoassays for detection of cancer biomarkers, and (3) methods for targeted therapy and automatically adjusted drug delivery such as in experimental stroke and brain injury studies. As nanoproteomics becomes available both to the clinician at the bedside and the citizens who are increasingly interested in access to novel post-genomics diagnostics through initiatives such as the quantified self, we anticipate further breakthroughs in personalized and targeted medicine.
    Omics: a journal of integrative biology 01/2014; 18(2). DOI:10.1089/omi.2013.0074 · 2.36 Impact Factor
  • Source
    • ". Nanostructured CeO 2 has been used for immobilization of antibodies for ochratoxin detection [23]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Lipase has been immobilized onto sol–gel derived nanostuctured cerium oxide (Nano-CeO2, 35 nm) film deposited onto indium–tin–oxide (ITO) coated glass plate for tributyrin detection. The Nano-CeO2/ITO electrode and lipase/nano-CeO2/ITO bioelectrode have been characterized using scanning electron microscopy (SEM) and cyclic voltammetry (CV). The electrochemical response of lipase/nano-CeO2/ITO bioelectrode towards tributyrin, investigated using CV studies, exhibits linearity, detection limit and shelf life as 50–500 mg/dL, 32.8 mg/dL and 12 weeks, respectively. The value of the apparent Michaelis–Menten constant () obtained as 22.27 mg/dL (0.736 mM) for lipase/nano-CeO2/ITO bioelectrode indicates high affinity of lipase with tributyrin. Attempts have been made to detect triglyceride concentration in serum samples.
    Sensors and Actuators B Chemical 09/2009; 141(2-141):551-556. DOI:10.1016/j.snb.2009.05.034 · 4.10 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Self-assembled monolayers (SAMs) have aroused much interest due to their potential applications in biosensors, biomolecular electronics and nanotechnology. This has been largely attributed to their inherent ordered arrangement and controllable properties. SAMs can be formed by chemisorption of organic molecules containing groups like thiols, disulphides, amines, acids or silanes, on desired surfaces and can be used to fabricate biomolecular electronic devices. We focus on recent applications of organosulphur compounds (thiols) based SAMs to biomolecular electronic devices in the last about 3 years.
    Biosensors & Bioelectronics 03/2009; 24(9):2810-7. DOI:10.1016/j.bios.2009.02.008 · 6.41 Impact Factor
Show more