Conference Paper

AUXIN TRANSPORT AND RIBOSOME BIOGENESIS MUTANT / REPORTER LINES TO STUDY PLANT CELL GROWTH AND PROLIFERATION UNDER ALTERED GRAVITY

In proceeding of: Proceedings of the Joint Life Science Symposium, Aberdeen, United Kingdom, 18-22 June 2012.

ABSTRACT We tested different Arabidopsis thaliana strains to check their availability for space use in the International Space Station (ISS). We used mutants and reporter gene strains affecting factors of cell proliferation and cell growth, to check variations induced by an altered gravity vector. Seedlings were grown either in a Random Positioning Machine (RPM), under simulated microgravity (µg), or in a Large Diameter Centrifuge (LDC), under hypergravity (2g). A combination of the two devices (µg RPM+LDC) was also used. Under all gravity alterations, seedling roots were longer than in control 1g conditions, while the levels of the nucleolar protein nucleolin were depleted. Alterations in the pattern of expression of PIN2, an auxin transporter, and of cyclin B1, a cell cycle regulator, were shown. All these alterations are compatible with previous space data, so the use of these strains will be useful in the next experiments in ISS, under real microgravity.

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  • Strain µgRPM vs 1gRPM µgRPM+LDC 2gLDC vs 1gLDC Root length Wt + +* +* eir1.1 +* +* + atnuc-L1 + +* + Nucleolar size Wt -* - -* eir1.1 -* + + Protein expression pattern PIN2:GFP - -- --- ATNUC-L1. J Haseloff . 2122-2127.

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May 21, 2014