Article

Micrococcus endophyticus sp. nov., isolated from surface-sterilized Aquilaria sinensis roots.

The Key Laboratory for Microbial Resources of the Ministry of Education, PR China.
International Journal of Systematic and Evolutionary Microbiology (Impact Factor: 2.8). 06/2009; 59(Pt 5):1070-5. DOI: 10.1099/ijs.0.006296-0
Source: PubMed

ABSTRACT A Gram-positive bacterial strain, designated YIM 56238(T), was isolated from plant roots (Aquilaria sinensis), and characterized by using a polyphasic approach. Strain YIM 56238(T) grew optimally at pH 7.0-8.0 and at 28 degrees C. Analysis of the 16S rRNA gene sequence of strain YIM 56238(T) indicated that it belongs to the genus Micrococcus. Chemotaxonomic data strongly supported the classification of this strain within the genus Micrococcus: the cell-wall peptidoglycan contained lysine, glutamic acid, alanine and glycine; the predominant menaquinones were MK-8(H(2)) (63.6 %) and MK-7(H(2)) (21.1 %); the phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown ninhydrin-negative phospholipid; and the major cellular fatty acids were iso-C(15 : 0) (30.95 %) and anteiso-C(15 : 0) (53.75 %). The G+C content of the genomic DNA was 72.9 mol%. A number of physiological features were found that clearly distinguished strain YIM 56238(T) from recognized species of the genus Micrococcus. DNA-DNA hybridization studies suggested that the novel strain represents a separate genomic species. On the basis of the data, therefore, strain YIM 56238(T) represents a novel species of the genus Micrococcus, for which the name Micrococcus endophyticus sp. nov. is proposed. The type strain is YIM 56238(T) (=DSM 17945(T)=KCTC 19156(T)).

Download full-text

Full-text

Available from: Wen-Jun Li, Jun 13, 2015
0 Followers
 · 
290 Views
  • Source
    • "Since the first description of the taxon by Cohn in 1872, it has repeatedly been revised (Kokur et al. 1975; Stackebrandt et al. 1995) with the last amended and still valid classification done by Wieser et al. (2002). Accordingly, the ten Micrococcus species are: M. luteus (the type species of the genus, with three biovars; Cohn 1872; Wieser et al. 2002), M. lylae (Kloos et al. 1974; Wieser et al. 2002), M. antarcticus (Liu et al. 2000), M. flavus (Liu et al. 2007), M. endophyticus (Chen et al. 2009), M. yunnanensis (Zhao et al. 2009), M. terreus (Zhang et al. 2010), M. lactis (Chittpurna et al. 2011), M. niistensis (Dastager et al. 2010a), and M. cohnii (Rieser et al. 2012). herbicides, dyes, polycyclic aromatic hydrocarbons, polyacrylonitrile polymers, phthalate esters, and chlorinated biphenyls (Bevinakatti and Ninnekar 1993; Doddamani and Ninnekar 2001; Du et al. 2011; Eaton and Ribbons 1982; El-Sayeda et al. 2005; Fischer-Colbrie et al. 2007; Mulla et al. 2011; Rajee and Patterson 2011; Saratale et al. 2009; Sims et al. 1986; Tallur et al. 2008; Zheng et al. 2009; Zhuang et al. 2003a, b). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Micrococci are Gram-positive G + C-rich, nonmotile, nonspore-forming actinomycetous bacteria. Micrococcus comprises ten members, with Micrococcus luteus being the type species. Representatives of the genus play important roles in the biodegradation of xenobiotics, bioremediation processes, production of biotechnologically important enzymes or bioactive compounds, as test strains in biological assays for lysozyme and antibiotics, and as infective agents in immunocompromised humans. The first description of plasmids dates back approximately 28 years, when several extrachromosomal elements ranging in size from 1.5 to 30.2 kb were found in Micrococcus luteus. Up to the present, a number of circular plasmids conferring antibiotic resistance, the ability to degrade aromatic compounds, and osmotolerance are known, as well as cryptic elements with unidentified functions. Here, we review the Micrococcus extrachromosomal traits reported thus far including phages and the only quite recently described large linear extrachromosomal genetic elements, termed linear plasmids, which range in size from 75 kb (pJD12) to 110 kb (pLMA1) and which confer putative advantageous capabilities, such as antibiotic or heavy metal resistances (inferred from sequence analyses and curing experiments). The role of the extrachromosomal elements for the frequently proven ecological and biotechnological versatility of the genus will be addressed as well as their potential for the development and use as genetic tools.
    Applied Microbiology and Biotechnology 11/2012; 97(1). DOI:10.1007/s00253-012-4539-5 · 3.81 Impact Factor
  • Source
    • "At the time of writing, the genus Micrococcus comprised five species, Micrococcus luteus (Cohn, 1872), Micrococcus lylae (Kloos et al., 1974), Micrococcus antarcticus (Liu et al., 2000), Micrococcus flavus (Liu et al., 2007) and Micrococcus endophyticus (Chen et al., 2009). Members of the genus Micrococcus are aerobic, Gram-positive and catalasepositive . "
    [Show abstract] [Hide abstract]
    ABSTRACT: In this study, strain YIM 65004(T), isolated from roots of Polyspora axillaris, was shown to represent a novel species of the genus Micrococcus by means of a polyphasic approach. Chemotaxonomic data gathered for peptidoglycan type, menaquinones, phospholipids and fatty acids strongly supported the classification of this strain within the genus Micrococcus: the cell-wall peptidoglycan contained lysine, glutamic acid, alanine, glycine and aspartic acid, the predominant menaquinones were MK-8(H(2)) (66.97 %) and MK-7(H(2)) (23.26 %), the phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown ninhydrin-negative phospholipid, and the major cellular fatty acids were anteiso-C(15 : 0) (61.98 %), iso-C(16 : 0) (14.25 %) and iso-C(15 : 0) (13.04 %). The G+C content of the genomic DNA was 71.7 mol%. A number of physiological features were found that clearly distinguished strain YIM 65004(T) from recognized Micrococcus species. DNA-DNA hybridization studies suggested that the novel strain represents a separate genomic species. Based on the above data, a novel species of the genus Micrococcus, Micrococcus yunnanensis sp. nov., is proposed, with the type strain YIM 65004(T) (=CCTCC AA 208060(T)=DSM 21948(T)).
    International Journal of Systematic and Evolutionary Microbiology 08/2009; 59(Pt 10):2383-7. DOI:10.1099/ijs.0.010256-0 · 2.80 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Endophytic actinobacteria are relatively unexplored as potential sources of novel species and novel natural products for medical and commercial exploitation. Xishuangbanna is recognized throughout the world for its diverse flora, especially the rain forest plants, many of which have indigenous pharmaceutical histories. However, little is known about the endophytic actinobacteria of this tropical area. In this work, we studied the diversity of actinobacteria isolated from medicinal plants collected from tropical rain forests in Xishuangbanna. By the use of different selective isolation media and methods, a total of 2,174 actinobacteria were isolated. Forty-six isolates were selected on the basis of their morphologies on different media and were further characterized by 16S rRNA gene sequencing. The results showed an unexpected level of diversity, with 32 different genera. To our knowledge, this is the first report describing the isolation of Saccharopolyspora, Dietzia, Blastococcus, Dactylosporangium, Promicromonospora, Oerskovia, Actinocorallia, and Jiangella species from endophytic environments. At least 19 isolates are considered novel taxa by our current research. In addition, all 46 isolates were tested for antimicrobial activity and were screened for the presence of genes encoding polyketide synthetases and nonribosomal peptide synthetases. The results confirm that the medicinal plants of Xishuangbanna represent an extremely rich reservoir for the isolation of a significant diversity of actinobacteria, including novel species, that are potential sources for the discovery of biologically active compounds.
    Applied and Environmental Microbiology 08/2009; 75(19):6176-86. DOI:10.1128/AEM.01034-09 · 3.95 Impact Factor
Show more