Article

[A simple and efficient method for establishing a mouse model of orthotopic MB49 bladder cancer].

Department of Urologic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 05/2009; 29(4):627-30. pp.627-30
Source: PubMed

ABSTRACT To establish a simple and efficient method for establishing a mouse model of orthotopic superficial bladder cancer.
C57BL/6 mice were anesthetized with sodium pentobarbital and catheterized with modified IV catheter (24 G). The mice were intravesically pretreated with HCl and then with NaOH, and after washing the bladders with phosphate-buffered saline (PBS), 100 microl (1 x 10(7)) MB49 cells were infused and allowed to incubate in the bladder for 2 h followed intravesical mitomycin C (MMC) administration. The tumor formation rate, survival, gross hematuria, and bladder weight were determined as the outcome variables, and the pathology of the bladders was observed.
Instillation of MB49 tumor cells resulted in a tumor formation rates of 100% in all the pretreated groups while 0% in the control group without pretreatment. MMC significantly reduced the bladder weight as compared to PBS.
We have successfully established a stable, reproducible, and reliable orthotopic bladder cancer model in mice.

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Keywords

bladder weight
 
bladders
 
catheterized
 
control group
 
efficient method
 
gross hematuria
 
HCl
 
incubate
 
intravesical mitomycin C
 
IV catheter
 
MB49 tumor cells
 
mice
 
MMC
 
orthotopic superficial bladder cancer
 
outcome variables
 
reliable orthotopic bladder cancer model
 
reproducible
 
sodium pentobarbital
 
tumor formation rate
 
tumor formation rates