The cell wall and secretory proteome of a tobacco cell line synthesising secondary wall

School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, UK.
Proteomics (Impact Factor: 3.97). 05/2009; 9(9):2355-72. DOI: 10.1002/pmic.200800721
Source: PubMed

ABSTRACT The utility of plant secondary cell wall biomass for industrial and biofuel purposes depends upon improving cellulose amount, availability and extractability. The possibility of engineering such biomass requires much more knowledge of the genes and proteins involved in the synthesis, modification and assembly of cellulose, lignin and xylans. Proteomic data are essential to aid gene annotation and understanding of polymer biosynthesis. Comparative proteomes were determined for secondary walls of stem xylem and transgenic xylogenic cells of tobacco and detected peroxidase, cellulase, chitinase, pectinesterase and a number of defence/cell death related proteins, but not marker proteins of primary walls such as xyloglucan endotransglycosidase and expansins. Only the corresponding detergent soluble proteome of secretory microsomes from the xylogenic cultured cells, subjected to ion-exchange chromatography, could be determined accurately since, xylem-specific membrane yields were of poor quality from stem tissue. Among the 109 proteins analysed, many of the protein markers of the ER such as BiP, HSP70, calreticulin and calnexin were identified, together with some of the biosynthetic enzymes and associated polypeptides involved in polymer synthesis. However 53% of these endomembrane proteins failed identification despite the use of two different MS methods, leaving considerable possibilities for future identification of novel proteins involved in secondary wall polymer synthesis once full genomic data are available.

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    • "occurring primarily in Fabaceae that associate with nitrogen-fixing bacteria have been used in proteomics studies. Finally, extracellular fluids such as root exudates [142], xylem and phloem sap [80] [143] [144], and intercellular washing (apoplast) fluid [145] [146] have been analyzed at the protein level. "
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    ABSTRACT: The topic of plant proteomics is reviewed based on related papers published in the journal "Proteomics" since publication of the first issue in 2001. In total, around 300 original papers and 41 reviews published in "Proteomics" between 2000 and 2014 have been surveyed. Our main objective for this review is to help bridge the gap between plant biologists and proteomics technologists, two often very separate groups. Over the past years a number of reviews on plant proteomics have been published [1-7]. To avoid repetition we have focused on more recent literature published after 2010, and have chosen to rather make continuous reference to older publications. The use of the latest proteomics techniques, its integration with other approaches in the Systems Biology direction are discussed more in detail. Finally we comment on the recent history, state of the art, and future directions of plant proteomics, using publications in "Proteomics" to illustrate the progress in the field. The review is organized into two major blocks, the first devoted to provide an overview of experimental systems (plants, plant organs, biological processes) and the second one to the methodology. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
    Proteomics 03/2015; 15(5-6). DOI:10.1002/pmic.201400349 · 3.97 Impact Factor
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    • "Due to these possible interactions, many technical difficulties are encountered during extraction and analysis of cell wall proteins. Moreover, accessibility and availability of CWPs from specific tissues in a living plant are not always straightforward, making the analysis experimentally limited [11]. Hence, tissue culture systems provide a convenient and attractive source of cell wall proteins. "
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    Plant Physiology and Biochemistry 11/2012; 62C:1-10. DOI:10.1016/j.plaphy.2012.10.015 · 2.35 Impact Factor
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    • "Until now, the best well characterized cell wall proteome is that of Arabidopsis plant (Albenne et al. 2009; Bayer et al. 2006; Borderies et al. 2003; Chivasa et al. 2002; Jamet et al. 2006, 2008), but consistent data are also available, among others, on rice (Chen et al. 2009; Cho et al. 2009), maize (Zhu et al. 2006, 2007), medicago (Watson et al. 2004) and tobacco (Blee et al. 2001; Millar et al. 2009). The main part of these data was obtained from stems, roots, leaves or calli analysis. "
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    Journal of Plant Research 08/2012; 126. DOI:10.1007/s10265-012-0516-4 · 2.51 Impact Factor
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