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Available from: Gary Siuzdak, Jan 16, 2014
    • "Another approach is based on cellular reprogramming with proteins as reprogramming factors. As a proof-of-principle, MEF cells were reprogrammed using a fusion protein which contained the four reprogramming factors with a poly-arginine protein transduction domain [92]. A new alternative based on recombinant proteins from an Escherichia coli expression system was used on murine and human fibroblasts. "
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    ABSTRACT: Adult cells from patients can be reprogrammed to induced pluripotent stem cells (iPSCs) which successively can be used to obtain specific cells such as neurons. This remarkable breakthrough represents a new way of studying diseases and brought new therapeutic perspectives in the field of Regenerative Medicine. This is particular true in the neurology field, where few techniques are amenable to study the affected tissue of the patient during disease progression and many diseases are lacking neuroprotective therapies or any therapy at all. In this review we discuss the advantages and unresolved issues of cell reprogramming and neuronal differentiation. We reviewed evidence using iPSCs-derived neurons from neurological patients. Focusing on data obtained from Parkinson's disease (PD) patients, we show that iPSC-derived neurons possess morphological and functional characteristics of this disease and build a case for the use of this technology to study PD and other neuropathologies while disease is in progress. These data show the enormous impact that this new technology starts to have on different purposes such as the study and design of future therapies of neurological disease, especially PD. Copyright © 2015. Published by Elsevier B.V.
    FEBS letters 07/2015; 589(22). DOI:10.1016/j.febslet.2015.07.023 · 3.17 Impact Factor
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    • "This has prompted research into suitable alternatives to viral and plasmid reprogramming (Okita et al., 2008; Woltjen et al., 2009; Yu et al., 2009; Zhou et al., 2009; Li et al., 2011; Worsdorfer et al., 2013; Talluri et al., 2014). The SB and PB transposon systems share many advantages for mediating epigenetic reprogramming, and possess a high cargo capacity of >100 kb (Rostovskaya et al., 2012). "
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    ABSTRACT: Induced pluripotent stem cells (iPSCs) are a seminal breakthrough in stem cell research and are promising tools for advanced regenerative therapies in humans and reproductive biotechnology in farm animals. iPSCs are particularly valuable in species in which authentic embryonic stem cell (ESC) lines are yet not available. Here, we describe a nonviral method for the derivation of bovine iPSCs employing Sleeping Beauty (SB) and piggyBac (PB) transposon systems encoding different combinations of reprogramming factors, each separated by self-cleaving peptide sequences and driven by the chimeric CAGGS promoter. One bovine iPSC line (biPS-1) generated by a PB vector containing six reprogramming genes was analyzed in detail, including morphology, alkaline phosphatase expression, and typical hallmarks of pluripotency, such as expression of pluripotency markers and formation of mature teratomas in immunodeficient mice. Moreover, the biPS-1 line allowed a second round of SB transposon-mediated gene transfer. These results are promising for derivation of germ line-competent bovine iPSCs and will facilitate genetic modification of the bovine genome.
    Cellular Reprogramming 04/2015; DOI:10.1089/cell.2014.0080. · 1.79 Impact Factor
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    • "Even the seemingly straightforward DNA-free non-integrating methods [e.g. small molecule cocktails (Hou et al, 2013) or proteins (Zhou et al, 2009)] remain highly inefficient, requiring intricate protocols with multiple rounds of treatment, large doses, and uncontrolled presence/kinetics of reprogramming factors. Thus, new strategies are required to overcome the limitations associated with these traditional reprogramming methods. "
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    ABSTRACT: Derived from any somatic cell type and possessing unlimited self-renewal and differentiation potential, induced pluripotent stem cells (iPSCs) are poised to revolutionize stem cell biology and regenerative medicine research, bringing unprecedented opportunities for treating debilitating human diseases. To overcome the limitations associated with safety, efficiency, and scalability of traditional iPSC derivation, expansion, and differentiation protocols, biomaterials have recently been considered. Beyond addressing these limitations, the integration of biomaterials with existing iPSC culture platforms could offer additional opportunities to better probe the biology and control the behavior of iPSCs or their progeny in vitro and in vivo. Herein, we discuss the impact of biomaterials on the iPSC field, from derivation to tissue regeneration and modeling. Although still exploratory, we envision the emerging combination of biomaterials and iPSCs will be critical in the successful application of iPSCs and their progeny for research and clinical translation. © 2015 The Authors.
    The EMBO Journal 03/2015; 34(8). DOI:10.15252/embj.201490756 · 10.43 Impact Factor
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