Article

C-jun N-terminal kinase-mediated signaling is essential for Staphylococcus aureus-induced U937 apoptosis.

Department of Geriatrics, Shengjing Hospital, China Medical University, Shenyang 110004, China.
Chinese Medical Sciences Journal 04/2009; 24(1):26-9. pp.26-9
Source: PubMed

ABSTRACT To investigate the effect of SP600125, a specific c-jun N-terminal protein kinase (JNK) inhibitor, on Staphylococcus aureus (S. aureus)-induced U937 cell death and the underlying mechanism.
The human monocytic U937 cells were treated with S. aureus at different time with or without SP600125. Cell apoptosis was analyzed by flow cytometry. JNK, Bax, and caspase-3 activities were detected by Western blotting.
S. aureus induced apoptosis in cultured U937 cells in a time-dependent manner. Expression of Bax and phospho-JNK significantly increased in S. aureus-treated U937 cells, and the level of activated caspase-3 also increased in a time-dependent manner. Inhibition of JNK with SP600125 significantly inhibited S. aureus-induced apoptosis in U937 cells.
S. aureus can induce apoptosis in U937 cells by phosphorylation of JNK and activation of Bax and caspase-3. SP600125 protects U937 cells from apoptosis induced by S. aureus via inhibiting the activity of JNK.

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Keywords

activated caspase-3
 
apoptosis induced
 
Bax
 
caspase-3
 
caspase-3 activities
 
Cell apoptosis
 
cultured U937 cells
 
different time
 
flow cytometry
 
human monocytic U937 cells
 
Inhibition
 
phospho-JNK
 
S. aureus
 
S. aureus induced apoptosis
 
S. aureus-treated U937 cells
 
specific c-jun N-terminal protein kinase
 
Staphylococcus aureus
 
time-dependent manner
 
underlying mechanism
 
Western blotting
 

Jia-He Wang