Article

Single-molecule FRET analysis of protein-DNA complexes.

Department of Physics and IRC in Bionanotechnology, Clarendon Laboratory, University of Oxford, Parks Road, Oxford, OX1 3PU, UK.
Methods in molecular biology (Clifton, N.J.) 02/2009; 543:503-21. DOI:10.1007/978-1-60327-015-1_29 pp.503-21
Source: PubMed

ABSTRACT We present a single-molecule method for studying protein-DNA interactions based on fluorescence resonance energy transfer (FRET) and alternating-laser excitation (ALEX) of single diffusing molecules. An application of this method to the study of a bacterial transcription initiation complex is presented.

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Keywords

alternating-laser excitation
 
bacterial transcription initiation complex
 
fluorescence resonance energy transfer
 
protein-DNA interactions
 
single diffusing molecules
 
single-molecule method