Inflammatory cytokine gene polymorphisms and spontaneous preterm birth.
ABSTRACT The objective was to search for an association between spontaneous preterm birth (sPTB) and single and/or combined polymorphisms in genes TNFA -308 G>A, IL10 -1082 G>A, IL10 -819 C>T, IL10 -592 C>A, IL6 -174 G>C, and IFNG +874 A>T. Genotyping was performed on 410 Brazilian ethnically matched women managed at two hospitals (two independent case-control sets). One set consisted of 122 cases and 101 controls, and the other set comprised 82 cases and 105 controls. We compared genotype and genotype-combination frequencies between cases and controls using Fisher's exact or the chi(2) tests and confirmed results using logistic regression. Among the six SNPs studied, we found no independent association between any single SNP and sPTB risk. The multi-locus analysis revealed a significant association between sPTB and the TNFA(GG)/IL6(GG)/IFNG(AA) genotype combination (p=0.002), confirmed by logistic regression. Our data suggest that the combination of TNF-alpha, IFN-gamma, and IL-6 maternal gene polymorphisms might contribute to susceptibility to sPTB. This finding could be investigated as a possible genetic marker for the risk of spontaneous preterm birth.
- SourceAvailable from: Bernardo BertoniPreterm Birth - Mother and Child, 01/2012; , ISBN: 978-953-307-828-1
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ABSTRACT: Bessel beams can be used as optical tweezers, to trap and manipulate small particles, including biological specimens. Here we demonstrate the use of such beams to trap and manipulate particles simultaneously that may reside in completely separate sample chambers, separated by distances that preclude trapping with a Gaussian beam. This also demonstrates another property of the Bessel beam, in that since it is a set of rings it can trap both low and high refractive index particles. The distance behind the particle that the Bessel beam reconstructs is dependent on the properties of the particle, and this may be useful in cell characterisation. We also demonstrate the generation of more complex patterns of nondiffracting light beams, by using interfering Bessel beams. We generate these by using a Mach-Zender interferometer in which each of the arms has a Laguerre-Gaussian beam of differing handedness.
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ABSTRACT: Objective: To investigate the association between preterm birth and cytokine genes (IFN-γ, interleukin (IL)-10) in intrauterine infection and enzyme gene (CYP1A1) in oxidative stress response. Methods: This study involved a case-control study conducted at Ewha Womans University Hospital in Seoul, Korea. Subjects with preterm deliveries (<37 weeks of gestation) and normal controls with term deliveries (≥37 weeks of gestation) were selected from gravidas who had undergone prenatal examinations in the hospital and were followed until infant delivery. The weight, height, and blood samples of each participants were obtained according to standard protocols. We included subjects who gave birth to a singleton infant and had a gestational age between 24 and 42 weeks. Mutiple births, stillbirths, and congenital anomalies were excluded. Finally, 164 gravidas with preterm births and 305 normal controls with term deliveries were enrolled in the present study. Results: Preterm delivery group and term delivery group had significant difference in gestational age and neonatal body weight (P<0.0001). There were no statistically significant association between preterm birth and IFN-γ, IL-10, CYP1A1 genes (P>0.05). Conclusion: In this study, IFN-γ (874A/T), IL-10 (1082A/G), IL-10 (819C/T), IL-10 (592A/C) and CYP1A1 (T6235C), CYP1A1 (Ile462val [A/G]) genes had no significant association with preterm birth.01/2010; 53(7). DOI:10.5468/kjog.2010.53.7.608