Article

Targeted gold nanoparticles enable molecular CT imaging of cancer.

Department of Chemistry, University of Michigan, Ann Arbor, USA.
Nano Letters (Impact Factor: 12.94). 01/2009; 8(12):4593-6. DOI: 10.1021/nl8029114
Source: PubMed

ABSTRACT X-ray based computed tomography (CT) is among the most convenient imaging/diagnostic tools in hospitals today in terms of availability, efficiency, and cost. However, in contrast to magnetic resonance imaging (MRI) and various nuclear medicine imaging modalities, CT is not considered a molecular imaging modality since targeted and molecularly specific contrast agents have not yet been developed. Here we describe a targeted molecular imaging platform that enables, for the first time, cancer detection at the cellular and molecular level with standard clinical CT. The method is based on gold nanoprobes that selectively and sensitively target tumor selective antigens while inducing distinct contrast in CT imaging (increased X-ray attenuation). We present an in vitro proof of principle demonstration for head and neck cancer, showing that the attenuation coefficient for the molecularly targeted cells is over 5 times higher than for identical but untargeted cancer cells or for normal cells. We expect this novel imaging tool to lead to significant improvements in cancer therapy due to earlier detection, accurate staging, and microtumor identification.

2 Bookmarks
 · 
101 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: As an intensely studied computed tomography (CT) contrast agent, gold nanoparticle has been suggested to be combined with fluorescence imaging modality to offset the low sensitivity of CT. However, the strong quenching of gold nanoparticle on fluorescent dyes requires complicated design and shielding to overcome. Herein, we report a unique nanoprobe (M-NPAPF-Au) co-loading an aggregation-induced emission (AIE) red dye and gold nanoparticles into DSPE-PEG2000 micelles for dual-modal fluorescence/CT imaging. The nanoprobe was prepared based on a facile method of “one-pot ultrasonic emulsification”. Surprisingly, in the micelles system, fluorescence dye (NPAPF) efficiently overcame the strong fluorescence quenching of shielding-free gold nanoparticles and retained the crucial AIE feature. In vivo studies demonstrated the nanoprobe had superior tumor-targeting ability, excellent fluorescence and CT imaging effects. The totality of present studies clearly indicates the significant potential application of M-NPAPF-Au as a dual-modal non-invasive fluorescence/X-ray CT nanoprobe for in vivo tumor-targeted imaging and diagnosis.
    Biomaterials 02/2015; 42. · 8.31 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Cancer is a major threat to public health and is still one of the leading causes of death worldwide. Cancer biomarkers are extremely important in the process of early detection of cancer, diagnosis, judgment of the curative effect and the prognosis. They also play an important role in mechanistic research into carcinogenesis. Detection methods based on cancer biomarkers for the determination of cancer can directly affect the diagnosis and treatment. Therefore, the development of highly sensitive and selective approaches for the detection of cancer biomarkers is critical. Micro- and nanoparticles play an important role in the clinical detection of cancer biomarkers. In this review, we provide an overview of the commonly used clinical detection methods for cancer biomarkers, such as enzyme-linked immunosorbent assay, chemiluminescence, electrochemiluminescence, as well as their technical characteristics. Additionally, various applications of recent advances in microparticles and nanoparticles in the clinical detection of cancer biomarkers are also reviewed.
    Analytical methods 01/2013; 5(21):5862. · 1.94 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A novel x-ray fluorescence imaging setup for the in vivo detection of high-Z tracer distributions is investigated for its application in molecular imaging. The setup uses an energy resolved detection method based on a Bragg reflecting analyzer array together with a multiple scatter reducing radial collimator. The aim of this work is to investigate the potential application of this imaging method to in vivo imaging in humans. A proof of principle experiment modeling a partial setup for the detection of gold nano-particles was conducted in order to test the feasibility of the proposed imaging method. Furthermore a Monte Carlo simulation of the complete setup was created in order to quantify the dependence of the image quality on the applied radiation dose and on the geometrical collimator parameters as well as on the analyzer crystal parameters. The Monte Carlo simulation quantifies the signal-to-noise ratio per radiation dose and its dependence on the collimator parameters. Thereby the parameters needed for a dose efficient in vivo imaging of gold nano-particle based tracer distributions are quantified. However also a number of problems are found like the fluorescence emission as well as scatter from the collimator material obscuring the tracer fluorescence and the potentially large scan time.
    SPIE Medical Imaging; 03/2014

Preview

Download
6 Downloads
Available from