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    ABSTRACT: Fifteen light-horse mares were monitored daily by palpation and ultrasonography for one oestrous cycle and then randomly divided into two treatment groups. Five mares were treated with 2 litres of normal ovariectomised mare plasma (OVX) on Day 10 after ovulation and 10 mares received 2 litres anti-inhibin plasma (a-INH) beginning on Day 10 after ovulation. Mares receiving OVX plasma all had single ovulations after treatment, while 5 of the 10 mares receiving a-INH plasma had multiple ovulations (mean 1.6 ± 0.2, range 1–3). No significant effect of a-INH plasma treatment was detected in plasma concentrations of FSH, oestradiol or progesterone. These results demonstrate that passive immunisation of mares with a-INH plasma can induce multiple ovulations, and thus eliminate the need for daily gonadotrophin treatment or a prolonged series of active inoculations against inhibin.
    Equine Veterinary Journal 06/2010; 25(S15):103 - 106. · 2.29 Impact Factor
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    ABSTRACT: In the present study, 638 embryo transfers conducted over 3 yr were retrospectively examined to determine which factors (recipient, embryo and transfer) significantly influenced pregnancy and embryo loss rates and to determine how rates could be improved. On Day 7 or 8 after ovulation, embryos (fresh or cooled/transported) were transferred by surgical or nonsurgical techniques into recipients ovulating from 5 to 9 d before transfer. At 12 and 50 d of gestation (Day 0 = day of ovulation), pregnancy rates were 65.7% (419 of 638) and 55.5% (354 of 638). Pregnancy rates on Day 50 were significantly higher for recipients that had excellent to good uterine tone or were graded as "acceptable" during a pretransfer examination, usually performed 5 d after ovulation, versus recipients that had fair to poor uterine tone or were graded "marginally acceptable." Embryonic factors that significantly affected pregnancy rates were morphology grade, diameter and stage of development. The incidence of early embryonic death was 15.5% (65 of 419) from Days 12 to 50. Embryo loss rates were significantly higher in recipients used 7 or 9 d vs 5 or 6 d after ovulation. Embryos with minor morphological changes (Grade 2) resulted in more (P<0.05) embryo death than embryos with no morphological abnormalities (Grade 1). Between Days 12 and 50, the highest incidence of embryo death occurred during the interval from Days 17 to 25 of gestation. Embryonic vesicles that were imaged with ultrasound during the first pregnancy exam (5 d after transfer) resulted in significantly fewer embryonic deaths than vesicles not imaged until subsequent exams. In the present study, embryo morphology was predictive of the potential for an embryo to result in a viable pregnancy. Delayed development of the embryo upon collection from the donor or delayed development of the embryonic vesicle within the recipient's uterus was associated with a higher incidence of pregnancy failure. Recipient selection (age, day after ovulation, quality on Day 5) significantly affected pregnancy and embryo loss rates.
    Theriogenology 11/2000; 54(6):965-79. · 2.08 Impact Factor
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    ABSTRACT: The pattern of the main metabolite of prostaglandin (PG) F2α was recorded following a nonsurgical embryo transfer technique in 9 mares under field conditions in Estonia. Three patterns were observed. Two of them were characterised by PG release, thereas the third was not. A tendency towards a shortened cycle was seen in 3 mares. Observations were made regarding the manipulation of the uterus as being normal or difficult to perform. In general, mares where the procedure was considered difficult were also found to have a PG release.;
    Equine Veterinary Journal 01/1997; 29(4):286-289. · 2.29 Impact Factor

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