A High-Throughput Screening Method to Identify Potential Pesticides for Mosquito Control

Center for Medical, Agricultural, and Veterinary Entomology, USDA-ARS, 1600 SW 23rd Dr., Gainesville, FL 32608, USA.
Journal of Medical Entomology (Impact Factor: 1.95). 04/2009; 46(2):335-41. DOI: 10.1603/033.046.0219
Source: PubMed


Mosquitoes that transmit human diseases are of major importance to the international public health community. Pesticides remain a major component of integrated programs to control these medically important species. However, very few types of pesticides are currently registered for mosquito control. A high-throughput screening method using first-instar larvae of Aedes aegypti was created and evaluated in our laboratory to quickly screen large numbers of chemicals for activity against mosquitoes. LC50 values of a representative group of compounds were determined using this high-throughput screening method and compared with LD50 values determined by topical application against female adults of Ae. aegypti. Our results show that this high-throughput screening method is suitable for screening large numbers of candidate chemicals quickly to identify effective compounds.

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Available from: Julia Pridgeon, Jun 20, 2014
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    • "They include abamectin, ivermectin and doramectin, which are highly effective against a broad spectrum of common pests in agriculture, making avermectins one of the most widely used classes of parasiticides (Wislocki et al., 1989). Avermectins have also been reported to be effective veterinary drug and mosquito control agents (Tišler and Eržen, 2006; Pridgeon et al., 2009). The avermectin abamectin is a mixture that contains about 80% avermectin B1a and 20% avermectin B1b, which have similar biological and toxicological properties (Campbell, 1989). "
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    • "Bioassays were conducted to test the P. frutescens essential oils and pure principal compounds for their larvicidal activity against A. aegypti by using the bioassay system described by Pridgeon et al. (2009). Five 1-day-old A. aegypti larvae were added in a droplet of water to each well of 24-well plates (BD Labware, Franklin Lakes, NJ) by using a disposable 22.5 cm Pasteur pipette. "
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    • "Spinosad is a natural product that has been reported to be effective in killing mosquito larvae (Bond et al. 2004, Cetin et al. 2005, Darriet and Corbel 2006, Romi et al. 2006, Perez et al. 2007, Jiang and Mulla 2009, Pridgeon et al. 2009, Sadanandane et al. 2009, Hertlein et al. 2010). Our study using a single application of Natular XRT tablet containing 6.25% spinosad showed that larvae were significantly reduced for 5 wk in catch basins that received a total of 3.96 in. "
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