A High-Throughput Screening Method to Identify Potential Pesticides for Mosquito Control

Center for Medical, Agricultural, and Veterinary Entomology, USDA-ARS, 1600 SW 23rd Dr., Gainesville, FL 32608, USA.
Journal of Medical Entomology (Impact Factor: 1.95). 04/2009; 46(2):335-41. DOI: 10.1603/033.046.0219
Source: PubMed


Mosquitoes that transmit human diseases are of major importance to the international public health community. Pesticides remain a major component of integrated programs to control these medically important species. However, very few types of pesticides are currently registered for mosquito control. A high-throughput screening method using first-instar larvae of Aedes aegypti was created and evaluated in our laboratory to quickly screen large numbers of chemicals for activity against mosquitoes. LC50 values of a representative group of compounds were determined using this high-throughput screening method and compared with LD50 values determined by topical application against female adults of Ae. aegypti. Our results show that this high-throughput screening method is suitable for screening large numbers of candidate chemicals quickly to identify effective compounds.

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Available from: Julia Pridgeon, Jun 20, 2014
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    • "Bioassays were conducted to test chalcone and pyrazole compounds for their larvicidal activity against Ae. aegypti by using the bioassay system described earlier (Pridgeon et al., 2009). Five Ae. aegypti larvae were added in a droplet of water to each well of 24-well plates (BD Labware , Franklin Lakes, NJ) by use of a disposable 22.5-cm Pasteur pipette. "
    Medicinal Chemistry Research 10/2015; 24(10). DOI:10.1007/s00044-015-1415-8 · 1.40 Impact Factor
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    • "They include abamectin, ivermectin and doramectin, which are highly effective against a broad spectrum of common pests in agriculture, making avermectins one of the most widely used classes of parasiticides (Wislocki et al., 1989). Avermectins have also been reported to be effective veterinary drug and mosquito control agents (Tišler and Eržen, 2006; Pridgeon et al., 2009). The avermectin abamectin is a mixture that contains about 80% avermectin B1a and 20% avermectin B1b, which have similar biological and toxicological properties (Campbell, 1989). "
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    ABSTRACT: The aim of the present study was to evaluate the toxicity of abamectin to the neotropical cladoceran Ceriodaphnia silvestrii. To this end, acute and chronic bioassays were conducted with the commercial formulation Vertimec® 18 EC. In addition, the toxicity of water samples taken from a microcosm experiment evaluating the effects of a single application (144μga.i./L) and two applications (2×36μga.i./L) of Vertimec® 18 EC, in the presence or absence of a tadpole species (Lithobates catesbeianus), was also assessed. The acute LC50-48h for immobilization was 1.47μga.i./L and chronic NOEC-8d for survival and fertility (number of neonates per female) were 169 and 84nga.i./L, respectively. Irrespective of the presence of tadpoles, water samples from the microcosms applied with the single concentration of 144μga.i./L remained toxic until the end of the experiment, even when samples were diluted 32 times with culture medium. Water in the repeated pesticide treatment showed a similar toxic response after both applications. Toxicity of water samples from the microcosms was lower than that expected based on the generated LC50 values, which is explained by a potential reduced bioavailability of the test compound resulting from absorbance to organic material. Potential side-effects on C. silvestrii related with the use of Vertimec® 18 EC in Brazil and the suitability of this species for tropical toxicity testing are discussed. Copyright © 2015. Published by Elsevier Ltd.
    Chemosphere 08/2015; 139:558-564. DOI:10.1016/j.chemosphere.2015.08.006 · 3.34 Impact Factor
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    • "Bioassays were conducted to test the P. frutescens essential oils and pure principal compounds for their larvicidal activity against A. aegypti by using the bioassay system described by Pridgeon et al. (2009). Five 1-day-old A. aegypti larvae were added in a droplet of water to each well of 24-well plates (BD Labware, Franklin Lakes, NJ) by using a disposable 22.5 cm Pasteur pipette. "
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    ABSTRACT: Dengue fever is a mosquito-borne disease transmitted by Aedes aegypti producing high rates of human morbidity and mortality. In order to find new and effective compounds against A. aegypti with low mammalian and less environmental toxic products, green and red Perilla frutescens (L.) Britt. (perilla) essential oils were investigated for their deterrent and larvicidal activity. Green perilla oil showed more promising deterrent and larvicidal activity than red perilla oil. Therefore, we focused on the principle compounds in green perilla oil and, in addition, perilla alcohol and perillic acid were included for the activity relationship on the allylic methyl groups on carbon 7. Chemical composition of green and red perilla essential oils was compared using gas-chromatography–flame ionization detection (GC–FID) and gas chromatography–mass spectrometry (GC–MS). In biting deterrent bioassays, biting deterrence index (BDI) values for compounds methyl perillate (0.73), perillic acid (0.71), perilla alcohol (0.69), perilla aldehyde (0.62), caryophyllene oxide (0.60), and limonene (0.52) indicated good biting deterrent activity but the activity was significantly lower (proportion not biting (PNB) value 0.84) than the positive control N,N-diethyl-3-methylbenzamide (DEET) at 25 nmol/cm2. In larval bioassays, methyl perillate was the most toxic compound with LC50 of 16.0 ppm. Methyl perillate was the most active compound against A. aegypti larvae and this compound may offer a new biodegradable mosquito control agent.
    Industrial Crops and Products 03/2015; 65. DOI:10.1016/j.indcrop.2014.11.043 · 2.84 Impact Factor
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