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Bedoui S, Whitney PG, Waithman J et al.Cross-presentation of viral and self antigens by skin-derived CD103+ dendritic cells. Nat Immunol 10:488-495

The Department of Microbiology and Immunology, The University of Melbourne, Parkville, Victoria, Australia.
Nature Immunology (Impact Factor: 24.97). 05/2009; 10(5):488-95. DOI: 10.1038/ni.1724
Source: PubMed

ABSTRACT Skin-derived dendritic cells (DCs) include Langerhans cells, classical dermal DCs and a langerin-positive CD103(+) dermal subset. We examined their involvement in the presentation of skin-associated viral and self antigens. Only the CD103(+) subset efficiently presented antigens of herpes simplex virus type 1 to naive CD8(+) T cells, although all subsets presented these antigens to CD4(+) T cells. This showed that CD103(+) DCs were the migratory subset most efficient at processing viral antigens into the major histocompatibility complex class I pathway, potentially through cross-presentation. This was supported by data showing only CD103(+) DCs efficiently cross-presented skin-derived self antigens. This indicates CD103(+) DCs are the main migratory subtype able to cross-present viral and self antigens, which identifies another level of specialization for skin DCs.

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Available from: Liv Eidsmo, Aug 28, 2015
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    • "The death of antigen-carrying skin-derived DCs would also be expected to accelerate antigen transfer to LN-resident DCs. The priming capability of CD103−DDCs in relation to CD4+ T cells is higher than for CD8+ T cells44, while CD8α+ LN-resident DCs are known to be effective antigen-presenting cells for CD8+ T cells34445, with high phagocytic activity46. Thus, it is possible that the influx of a large number of apoptotic antigen-carrying CD103−DDCs triggers accelerated phagocytosis and cross priming by CD8α+ LN-resident DCs. "
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    • "In the epidermis, Langerhans cells (LC) form a dense network of DC capable of capturing antigen and migrating to the LN after traversing the basement membrane into the dermis. In mice, LC appear to be particularly efficient at tolerance induction and the formation of regulatory T cells (Tregs) (6, 7), whereas they are dispensable for induction of CD8 T cell responses to infections (8–10). Skin macrophages also populate the dermis and include perivascular macrophages that are distributed along post-capillary venules and can assist with the recruitment of neutrophils from the blood (11). "
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    • "Here we report that BCL6 protein levels are high in steady-state migratory cDCs, but low in CD11cint I-Ahi cDCs after exposure to LPS or adjuvants, suggesting that the transcriptional repressor BCL6 might play a role in the induction of immune tolerance of self-antigens in the steady state. In line with this idea, the most intense BCL6 expression is observed in the steady-state splenic CD8α+ and LN CD103+ cDC subpopulations (data not shown), subsets reported to cross-present self-antigens from dead cells to induce self-tolerance [35], [36]. "
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