Hoogenkamp M, Lichtinger M, Krysinska H, Lancrin C, Clarke D, Williamson A et al.. Early chromatin unfolding by RUNX1: a molecular explanation for differential requirements during specification versus maintenance of the hematopoietic gene expression program. Blood 114: 299-309

Leeds Institute for Molecular Medicine, University of Leeds, Leeds, United Kingdom.
Blood (Impact Factor: 10.43). 05/2009; 114(2):299-309. DOI: 10.1182/blood-2008-11-191890
Source: PubMed

ABSTRACT At the cellular level, development progresses through successive regulatory states, each characterized by their specific gene expression profile. However, the molecular mechanisms regulating first the priming and then maintenance of gene expression within one developmental pathway are essentially unknown. The hematopoietic system represents a powerful experimental model to address these questions and here we have focused on a regulatory circuit playing a central role in myelopoiesis: the transcription factor PU.1, its target gene colony-stimulating-factor 1 receptor (Csf1r), and key upstream regulators such as RUNX1. We find that during ontogeny, chromatin unfolding precedes the establishment of active histone marks and the formation of stable transcription factor complexes at the Pu.1 locus and we show that chromatin remodeling is mediated by the transient binding of RUNX1 to Pu.1 cis-elements. By contrast, chromatin reorganization of Csf1r requires prior expression of PU.1 together with RUNX1 binding. Once the full hematopoietic program is established, stable transcription factor complexes and active chromatin can be maintained without RUNX1. Our experiments therefore demonstrate how individual transcription factors function in a differentiation stage-specific manner to differentially affect the initiation versus maintenance of a developmental program.

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Available from: Christophe Lancrin, Sep 23, 2014
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    • "Interestingly, the chromatin at this enhancer harbors active marks already in progenitors and is bound by PU.1 and IRF factors before Pax5 transcription takes place in committed pro-B cells (55). It was also shown that the concerted action of PU.1 and Runx1 primes the activation of both promoter and enhancer elements of the c-fms gene in myeloid cell (92, 93). All together, these data clearly indicate the pioneering and priming abilities of the master hematopoietic regulator PU.1. "
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    Frontiers in Immunology 04/2014; 5:156. DOI:10.3389/fimmu.2014.00156
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    • "As we suggested previously in the " cocktail party " model of differentiation (Sieweke & Graf, 1998), transcription factor complex formation is dynamic and differentiation stage specific. Runx1 thus forms complexes and cooperates with PU.1 during the initiation of c-fms expression but is dispensable at later stages of differentiation (Hoogenkamp et al., 2009), where Egr-2 cooperates with PU.1 (Krysinska et al., 2007). Similarly, at later stages of differentiation , the transcription factor IRF8 can cooperatively interact with DNA-bound PU.1 without binding to DNA itself and thereby enhances macrophage differentiation through targeted gene induction (Sharf et al., 1997; Tamura, Thotakura, Tanaka, Ko, & Ozato, 2005). "
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    Advances in Immunology 01/2013; 120:269-300. DOI:10.1016/B978-0-12-417028-5.00010-7 · 5.53 Impact Factor
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    • "Cebpa did not respond to RUNX1 induction, and neither did the known RUNX1 target Csf1r. As shown previously (Hoogenkamp et al, 2009), Csf1r cannot be bound by RUNX1 at this developmental stage (Supplementary Figure 6D). "
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    ABSTRACT: Cell fate decisions during haematopoiesis are governed by lineage-specific transcription factors, such as RUNX1, SCL/TAL1, FLI1 and C/EBP family members. To gain insight into how these transcription factors regulate the activation of haematopoietic genes during embryonic development, we measured the genome-wide dynamics of transcription factor assembly on their target genes during the RUNX1-dependent transition from haemogenic endothelium (HE) to haematopoietic progenitors. Using a Runx1-/- embryonic stem cell differentiation model expressing an inducible Runx1 gene, we show that in the absence of RUNX1, haematopoietic genes bind SCL/TAL1, FLI1 and C/EBPβ and that this early priming is required for correct temporal expression of the myeloid master regulator PU.1 and its downstream targets. After induction, RUNX1 binds to numerous de novo sites, initiating a local increase in histone acetylation and rapid global alterations in the binding patterns of SCL/TAL1 and FLI1. The acquisition of haematopoietic fate controlled by Runx1 therefore does not represent the establishment of a new regulatory layer on top of a pre-existing HE program but instead entails global reorganization of lineage-specific transcription factor assemblies.
    The EMBO Journal 10/2012; 31(22). DOI:10.1038/emboj.2012.275 · 10.75 Impact Factor
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