Article

Method of bioluminescence imaging for molecular imaging of physiological and pathological processes.

Department of Nuclear Medicine, University Hospital Leuven, Herestraat 49, Leuven B-3000, Belgium.
Methods (Impact Factor: 3.22). 04/2009; 48(2):139-45. DOI: 10.1016/j.ymeth.2009.03.013
Source: PubMed

ABSTRACT Molecular imaging has emerged as a powerful tool in basic, pre-clinical and clinical research for monitoring a variety of molecular and cellular processes in living organisms. Optical imaging techniques, mainly bioluminescence imaging, have extensively been used to study biological processes because of their exquisite sensitivity and high signal-to noise ratio. However, current applications have mainly been limited to small animals due to attenuation and scattering of light by tissues but efforts are ongoing to overcome these hurdles. Here, we focus on bioluminescence imaging by giving a brief overview of recent advances in instrumentation, current available reporter gene-reporter probe systems and applications such as cell trafficking, protein-protein interactions and imaging endogenous processes.

0 Followers
 · 
130 Views
  • Source
    • "Current in vivo microendoscopy imaging techniques include bioluminescence [2] and fluorescence techniques [3], and one task in the applications for quantitative microendoscopy image computing is that the motion in the microendoscopy image sequences needs to be corrected for better visualization and stable quantitative measures. Many methods such as image registration [4] [5] [6] were proposed for motion correction. "
    [Show abstract] [Hide abstract]
    ABSTRACT: In multimodality image-guided intervention for cancer diagnosis, a needle with cannula is first punctured using CT or MRI -guided system to target the tumor, then microendoscopy can be performed using an optical fiber through the same cannula. With real-time optical imaging, the operator can directly determine the malignance of the tumor or perform fine needle aspiration biopsy for further diagnosis. During this operation, stable microendoscopy image series are needed to quantify the tissue properties, but they are often affected by respiratory and heart systole motion even when the interventional probe is held steadily. This paper proposes a microendoscopy motion correction (MMC) algorithm using normalized mutual information (NMI)-based registration and a nonlinear system to model the longitudinal global transformations. Cubature Kalman filter is thus used to solve the underlying longitudinal transformations, which yields more stable and robust motion estimation. After global motion correction, longitudinal deformations among the image sequences are calculated to further refine the local tissue motion. Experimental results showed that compared to global and deformable image registrations, MMC yields more accurate alignment results for both simulated and real data.
    Medical Imaging and Augmented Reality - 5th International Workshop, MIAR 2010, Beijing, China, September 19-20, 2010. Proceedings; 01/2010
  • Methods 07/2009; 48(2):81-2. DOI:10.1016/j.ymeth.2009.05.012 · 3.22 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Mycobacterium tuberculosis, the causative agent of tuberculosis, still represents a major public health threat in many countries. Bioluminescence, the production of light by luciferase-catalyzed reactions, is a versatile reporter technology with multiple applications both in vitro and in vivo. In vivo bioluminescence imaging (BLI) represents one of its most outstanding uses by allowing the non-invasive localization of luciferase-expressing cells within a live animal. Despite the extensive use of luminescent reporters in mycobacteria, the resultant luminescent strains have not been fully applied to BLI. One of the main obstacles to the use of bioluminescence for in vivo imaging is the achievement of reporter protein expression levels high enough to obtain a signal that can be detected externally. Therefore, as a first step in the application of this technology to the study of mycobacterial infection in vivo, we have optimised the use of firefly, Gaussia and bacterial luciferases in mycobacteria using a combination of vectors, promoters, and codon-optimised genes. We report for the first time the functional expression of the whole bacterial lux operon in Mycobacterium tuberculosis and M. smegmatis thus allowing the development of auto-luminescent mycobacteria. We demonstrate that the Gaussia luciferase is secreted from bacterial cells and that this secretion does not require a signal sequence. Finally we prove that the signal produced by recombinant mycobacteria expressing either the firefly or bacterial luciferases can be non-invasively detected in the lungs of infected mice by bioluminescence imaging. While much work remains to be done, the finding that both firefly and bacterial luciferases can be detected non-invasively in live mice is an important first step to using these reporters to study the pathogenesis of M. tuberculosis and other mycobacterial species in vivo. Furthermore, the development of auto-luminescent mycobacteria has enormous ramifications for high throughput mycobacterial drug screening assays which are currently carried out either in a destructive manner using LuxAB or the firefly luciferase.
    PLoS ONE 05/2010; 5(5):e10777. DOI:10.1371/journal.pone.0010777 · 3.53 Impact Factor
Show more