Allergy to Salsola Kali in a Salsola Incanescens-rich Area: Role of Extensive Cross Allergenicity
ABSTRACT Pollens from the Salsola spp. are an important source of respiratory allergy in tropical countries. Our aim was to characterize the IgE binding proteins of S. incanescens pollen extract and study its cross-reactivity with S. kali pollen allergens.
Prick tests with S. kali and S. incanescens pollen extracts were performed on eight respiratory allergy patients from Mashhad, Northeast Iran. The antigenic profiles and IgE-binding patterns of S. kali and S. incanescens pollen extracts were compared by SDS-PAGE and Western blotting, using individual sera from the salsola pollen-sensitive patients. Cross-reactivity of proteins in the two weeds was assessed by IgE- immunoblotting inhibition.
S. kali and S. incanescens pollen extracts showed similar IgE-binding profiles in Western blotting. The IgE binding components of 39, 45, 66 and 85 kDa were detected in both pollen extracts. Furthermore, inhibition of the immunoblots revealed extensive inhibition of IgE binding to proteins and a close relationship between these two weeds allergens.
S. incanescens pollen is a potent allergen source with several IgE binding components that shows a close allergenic relationship with S. kali. Our results suggest that in S. incanescens-rich areas, S. kali pollen extracts could be used as a diagnostic reagent for allergic patients to S. incanescens pollen.
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ABSTRACT: Many pieces of evidence such as the synchraneity of seasonal variation in allergic symptoms with the rhythm of plant pollination suggest that pollen is one of the most probable causes of allergy. The aim of this study was the studying of pollens allergenicity in Chenopodium album and Chenopodium botrys. C. album and C. botrys grows commonly in different parts of the Iran. Pollens of these were collected from area of Tehran, Karaj city and around Kandovan. Pollens were extracted using phosphate-buffered saline, PH 7.4. Male guinea pigs were sensitized and treated with C.album and C. botrys pollen extracts and skin prick tests were performed on guinea pigs and quantified on the basis of wheal diameter. After treatment with pollen extracts, the guinea pigs blood was obtained directly from the heart and sera from samples were stored at-20ºc until analyzed. During the skin prick test, the allergenic sensitivity was observed for C.album pollen grains, with an average wheal diameter of about 4 cm and for C. botrys pollen grains, with an average wheal diameter of about 2/5 cm. Results of blood smears were seen that, the numbers of eosinophils, neutrophils and amount of IgE were increased in the animals treated with pollen extracts than in the control group.
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ABSTRACT: Ailanthus altissima or Ailanthus glandulosa (Simaroubaceae) is known as tree of heaven. It is a dioecious plant with staminate and pistillate flowers that grow on separate trees. In recent years, A. altissima has been frequently planted in numerous areas, especially in arid and semiarid lands of Iran and also used as an ornamental tree in several Iran cities including Kerman. The aim of this research was to identify IgE-binding proteins responsible for type I hypersensitivities of A. altissima pollen extract. In this study, pollen’s proteins were extracted and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Total protein content of pollen extracts was measured by Bradford assay. Allergenicity of pollen extract was evaluated by skin test and immunoblotting. Results showed that total protein concentration of A. altissima pollen was found to be 1.34 μg μl−1. In A. altissima pollen extract, different protein fractions were identified by SDS-PAGE mostly at 42, 53.7, 63, 87.7, 100 and 120 kDa. Skin tests showed a delayed hypersensitivity. Immunoblotting of A. altissima pollen extract with pooled subject’s sera detected a major IgE-binding component of 42 kDa. Moreover, these results will provide a platform for cloning cDNA encoding allergenic protein from A. altissima pollen.Aerobiologia 09/2013; 29(3). DOI:10.1007/s10453-013-9290-6 · 1.20 Impact Factor
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ABSTRACT: Asthma is a common chronic inflammatory of airways especially in childhood. Clinical manifestations include coughing, wheezing and sometimes dyspnea. Allergic rhinitis (AR) is a common nasal inflammatory disease in the world. Prevalence of AR is 10-40% in different regions of the world. Clinical manifestations of allergic rhinitis are sneezing, pruritus, congestion and rhino rhea. Etiologies of allergic disorders are multifactorial. Many patients with chronic urticaria are concerned about different allergens such as aeroallergens or food allergens that could induce or exacerbate their disease. Aeroallergens have an important role in exacerbation of allergic disorders especially asthma and allergic rhinitis, however, their role is less important in eczema and chronic urticaria. Several studies carried out in Iran using Skin Prick Test (a standard test for diagnosis of aeroallergens) showed involvement of pollens from grasses, weeds and trees in dry and warm climates but mites are more common in humid and warm climates such as north of Iran. J Mazand Univ Med Sci 2012; 22(87): 139-151 (Persian).