Article

Expression of CD27 and CD23 on peripheral blood B lymphocytes in humans of different ages.

Dipartimento di Medicina Clinica e Sperimentale, Sezione di Ematologia, Università di Verona, Italia.
Blood transfusion = Trasfusione del sangue (impact factor: 2.1). 02/2009; 7(1):29-34. DOI:10.2450/2008.0007-08 pp.29-34
Source: PubMed

ABSTRACT Due to the fact that the coexpression of CD23 and CD27 has been reported to occur in B lymphocytic leukaemic clones and that there is debate about CD23 expression on memory B cells, we evaluated the behaviour of naive B cells (CD23-/CD27-) and memory B cells (CD27+) in the peripheral blood of a large number of humans of all ages. B cells were also distinguished into B2 (CD5-) and B1-a cells (CD5+).
The cell surface expression of CD19, CD5, CD23 and CD27 was assessed on peripheral blood lymphocytes from 1,427 subjects of all ages undergoing peripheral blood immunophenotyping for a variety of reasons.
The absolute number of B lymphocytes and the percentage of naive cells (CD23-/CD27-) decreased with age whereas there was an increase in memory cells (CD27+). A small subset of B cells co-expressing CD23 and CD27 was present in humans of all ages, although the majority of CD27+ cells were CD23-. The percentages and rate of increase with age of B1-a CD23+/CD27+ were slightly higher than those of B2 cell counterparts.
On the basis of our data, age-associated changes in surface markers of B cells seem to be finely balanced and probably related to functional changes after antigen encounters, while the whole peripheral blood B-cell compartment undergoes a quantitative regression.

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Keywords

absolute number
 
age-associated changes
 
antigen encounters
 
B cells
 
B cells co-expressing CD23
 
B lymphocytes
 
B lymphocytic leukaemic clones
 
B1-a cells
 
B2 cell counterparts
 
CD23 expression
 
cell surface expression
 
finely balanced
 
functional changes
 
memory B cells
 
memory cells
 
naive B cells
 
naive cells
 
peripheral blood
 
peripheral blood lymphocytes
 
whole peripheral blood B-cell compartment undergoes