Article

Effects of creatine supplementation on oxidative stress and inflammatory markers after repeated-sprint exercise in humans

Laboratory of Nutrition and Metabolism, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil. Electronic address: .
Nutrition (Impact Factor: 3.05). 06/2013; 29(9). DOI: 10.1016/j.nut.2013.03.003
Source: PubMed

ABSTRACT The goal of this study was to evaluate the effects of creatine (Cr) supplementation on oxidative stress and inflammation markers after acute repeated-sprint exercise in humans.
Twenty-five players under age 20 y were randomly assigned to two groups: Cr supplemented and placebo. Double-blind controlled supplementation was performed using Cr (0.3 g/kg) or placebo tablets for 7 d. Before and after 7 d of supplementation, the athletes performed two consecutive Running-based Anaerobic Sprint Tests (RAST). RAST consisted of six 35-m sprint runs at maximum speed with 10 sec rest between them. Blood samples were collected just prior to start of test (pre), just after the completion (0 h), and 1 h after completion.
Average, maximum, and minimum power values were greater in the Cr-supplemented group compared with placebo (P < 0.05). There were significant increases (P < 0.05) in plasma tumor necrosis factor alpha (TNF-α) and C-reactive protein (CRP) up to 1 h after acute sprint exercise in the placebo-supplemented group. Malondialdehyde, lactate dehydrogenase (LDH), catalase, and superoxide dismutase enzymes also were increased after exercise in both groups. Red blood cell glutathione was lower after exercise in both groups. Cr supplementation reversed the increase in TNF-α and CRP as well as LDH induced by acute exercise. Controversially, Cr supplementation did not inhibit the rise in oxidative stress markers. Also, antioxidant enzyme activity was not different between placebo and Cr-supplemented groups.
Cr supplementation inhibited the increase of inflammation markers TNF-α and CRP, but not oxidative stress markers, due to acute exercise.

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    • "In the team-sport literature, there is a large diversity of run-based RSA protocols with differences in terms of sprint duration (4–6 s) or distance (10–40 m), sprint number (5– 15 repetitions), recovery time (10–30 s) or nature (passive or active) (Glaister et al. 2008; Spencer et al. 2005). While participant characteristics or sport-specific considerations complicate the emergence of 'gold standards' for the selection of RSA test, the popularity of the so-called Running Anaerobic Sprint Test or RAST (i.e. 6 × 35 m with 10 s rest) (Zagatto et al. 2009) has grown in recent years in both the scientific (Brocherie et al. 2014b; Cipryan and Gajda 2011; Deminice et al. 2013; Keir et al. 2013) and coaching (particularly in South America) communities. "
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