Article

Carotid repair using autologous adipose-derived endothelial cells.

Division of Cardiovascular Diseases, Mayo Clinic, Rochester, MN 55905, USA.
Stroke (impact factor: 5.73). 04/2009; 40(5):1886-91. DOI:10.1161/STROKEAHA.108.539932 pp.1886-91
Source: PubMed

ABSTRACT Adipose tissue is an abundant source of endothelial cells as well as stem and progenitor cells which can develop an endothelial phenotype. It has been demonstrated that these cells have distinct angiogenic properties in vitro and in vivo. However, whether these cells have the capacity to directly improve large vessel form and function after vascular injury remains unknown. To define whether delivery of adipose-derived endothelial cells (ADECs) would improve healing of injured carotid arteries, a rabbit model of acute arterial injury was used.
Autologous rabbit ADECs were generated using defined culture conditions. To test the ability of ADECs to enhance carotid artery repair, cells were delivered intraarterially after acute balloon injury. Additional delivery studies were performed after functional selection of cells before delivery.
After rabbit omental fat harvest and digestion, a proliferative, homogenous, and distinctly endothelial population of ADECs was identified. Direct delivery of autologous ADECs resulted in marked reendothelialization 48 hours after acute vascular injury as compared to saline controls (82.2+/-26.9% versus 4.2+/-3.0% P<0.001). Delivery of ADECs that were selected for their ability to take up acetylated LDL significantly improved vasoreactivity and decreased intimal formation after vascular injury.
Taken together, these data suggest that ADECs represent an autologous source of proliferative endothelial cells, which demonstrate the capacity to rapidly improve reendothelialization, improve vascular reactivity, and decrease intimal formation in a carotid artery injury model.

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Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

abundant source
 
acute arterial injury
 
acute balloon injury
 
acute vascular injury
 
Adipose tissue
 
adipose-derived endothelial cells
 
autologous ADECs
 
Autologous rabbit ADECs
 
autologous source
 
carotid artery
 
carotid artery injury model
 
decrease intimal formation
 
Direct delivery
 
endothelial cells
 
large vessel form
 
proliferative endothelial cells
 
rabbit model
 
reendothelialization 48 hours
 
saline controls
 
vascular injury
 

Harald Froehlich