Article

miR-29a suppresses tristetraprolin, which is a regulator of epithelial polarity and metastasis

IMBA Vienna, Institute for Molecular Pathology, Dr Bohrgasse 3-5, 1030 Vienna, Austria.
EMBO Reports (Impact Factor: 7.86). 03/2009; 10(4):400-5. DOI: 10.1038/embor.2009.9
Source: PubMed

ABSTRACT Several microRNAs (miRNAs) have recently been described as crucial regulators of epithelial-to-mesenchymal transition (EMT) and metastasis. By comparing the expression profiles of miRNAs, we found upregulation of miR-29a in mesenchymal, metastatic RasXT cells relative to epithelial EpRas cells. Overexpression of miR-29a suppressed the expression of tristetraprolin (TTP), a protein involved in the degradation of messenger RNAs with AU-rich 3'-untranslated regions, and led to EMT and metastasis in cooperation with oncogenic Ras signalling. We also observed enhanced miR-29a and reduced TTP levels in breast cancer patient samples, indicating relevance for human disease. Previously, miR-29 family members were shown to have tumour-suppressive effects in haematopoietic, cholangiocytic and lung tumours. Therefore, miRNAs can act as either oncogenes or tumour suppressors, depending on the context.

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    • "Interestingly, the miR-29b accounted for more than 83% of all miRNAs with this expression pattern. It has been shown that miR-29 plays diverse roles in different types of cancers (Garzon et al., 2009; Gebeshuber et al., 2009; Mott et al., 2007; Sengupta et al., 2008). Overexpression of miR-29 has been shown to interfere with bovine viral diarrhea virus (BVDV) replication (Fu et al., 2014b). "
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    ABSTRACT: The changes in cellular microRNA (miRNA) expression during the course of an adenovirus type 2 infection in human lung fibroblast were studied by deep RNA sequencing. Expressions of 175 miRNAs with over 100 transcripts per million nucleotides were changed more than 1.5-fold. The expression patterns of these miRNAs changed dramatically during the course of the infection, from upregulation of the miRNAs known as tumor suppressors (such as miR-22, miR-320, let-7, miR-181b, and miR-155) and down-regulation of oncogenic miRNAs (such as miR-21 and miR-31) early to downregulation of tumor suppressor miRNAs (such as let-7 family, mir-30 family, 23/27 cluster) and upregulation of oncogenic miRNAs (include miR-125, miR-27, miR-191) late after infection. The switch in miRNA expression pattern occurred when adenovirus DNA replication started. Furthermore, deregulation of cellular miRNA expression was a step-wise and special sets of miRNAs were deregulated in different phases of infection. Copyright © 2015 Elsevier Inc. All rights reserved.
    Virology 03/2015; 478C:99-111. DOI:10.1016/j.virol.2015.01.033 · 3.28 Impact Factor
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    • "Interestingly, the miR-29b accounted for more than 83% of all miRNAs with this expression pattern. It has been shown that miR-29 plays diverse roles in different types of cancers (Garzon et al., 2009; Gebeshuber et al., 2009; Mott et al., 2007; Sengupta et al., 2008). Overexpression of miR-29 has been shown to interfere with bovine viral diarrhea virus (BVDV) replication (Fu et al., 2014b). "
    [Show abstract] [Hide abstract]
    ABSTRACT: The changes in cellular microRNA (miRNA) expression during the course of an adenovirus type 2 infection in human lung fibroblast were studied by deep RNA sequencing. Expressions of 175 miRNAs with over 100 transcripts per million nucleotides were changed more than 1.5-fold. The expression patterns of these miRNAs changed dramatically during the course of the infection, from upregulation of the miRNAs known as tumor suppressors (such as miR-22, miR-320, let-7, miR-181b, and miR-155) and down-regulation of oncogenic miRNAs (such as miR-21 and miR-31) early to downregulation of tumor suppressor miRNAs (such as let-7 family, mir-30 family, 23/27 cluster) and upregulation of oncogenic miRNAs (include miR-125, miR-27, miR-191) late after infection. The switch in miRNA expression pattern occurred when adenovirus DNA replication started. Furthermore, deregulation of cellular miRNA expression was a step-wise and special sets of miRNAs were deregulated in different phases of infection
    Virology 03/2015; 478:99-111. · 3.28 Impact Factor
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    • "Apoptosis, cell cycle checkpoints, metastasis [39] [40] miR-29a Tristetraprolin EPH4, EPRAS, RASXT Metastasis [41] miR-16 BCL-2, CCND1, Caprin1, HMGA1 CDK6, CCNE1, CCND3, Survivin, WIP1 "
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    Clinical Biochemistry 12/2014; 48(6). DOI:10.1016/j.clinbiochem.2014.12.013 · 2.28 Impact Factor
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