Maritalea myrionectae gen. nov., sp. nov., isolated from a culture of the marine ciliate Myrionecta rubra. Int J Syst Evol Microbiol
ABSTRACT A Gram-negative, rod-shaped, strictly aerobic bacterium, designated strain CL-SK30(T), was isolated from a culture of the marine ciliate Myrionecta rubra. 16S rRNA gene sequence analysis revealed that strain CL-SK30(T) was most closely related to Cucumibacter marinus (92.0 % similarity) and next to the type strains of species of the genus Devosia (89.8-91.3 % similarities) in the family Hyphomicrobiaceae. Phylogenetic analyses of the 16S rRNA gene sequences showed that strain CL-SK30(T) formed a robust clade together with C. marinus, but the sequence divergence value of 8 % between them indicated that the novel bacterium represented a distinct lineage. Strain CL-SK30(T) grew optimally in the presence of 2-5 % sea salts at 30-35 degrees C and pH 7.2-8.0. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, two unidentified glycolipids, an unidentified phospholipid and an unidentified lipid. Ubiquinone 10 was the major quinone. The DNA G+C content was 52.7 mol%. Based on its phenotypic, chemotaxonomic and phylogenetic characteristics, strain CL-SK30(T) represents a novel genus and species of the family Hyphomicrobiaceae, for which the name Maritalea myrionectae gen. nov., sp. nov. is proposed. The type strain is CL-SK30(T) (=KCCM 90060(T)=DSM 19524(T)).
- [Show abstract] [Hide abstract]
ABSTRACT: Three Gram-negative, motile, aerobic bacteria were isolated from cultures of the marine red alga Porphyra yezoensis. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the novel strains were closely related to Maritalea myrionectae CL-SK30(T) (97.9% 16S rRNA gene sequence similarity) and Zhangella mobilis E6(T) (96.2 %). 16S rRNA gene sequence similarity between Z. mobilis E6(T) and M. myrionectae CL-SK30(T) was 97.9%. The DNA G+C contents of the isolates (49.4-50.0 mol%) were similar to those of M. myrionectae DSM 19524(T) (52.3 mol%) and Z. mobilis JCM 15144(T) (50.3 mol%). From these results, it was difficult to differentiate the genus Zhangella from the genus Maritalea. DNA-DNA hybridization demonstrated that the isolates belonged to a single species. The isolates could also be distinguished from M. myrionectae and Z. mobilis on the basis of chemotaxonomic and phenotypic features, including fatty acid composition (particularly C(16:1)ω7c), growth with 6-9% (w/v) NaCl, carbon utilization, oxidation patterns and so on. A novel species of the genus Maritalea is proposed to accommodate the three isolates, with the name Maritalea porphyrae sp. nov. The type strain is LCM-3(T) (=LMG 25872(T)=NBRC 107169(T)). Furthermore, it is proposed that Zhangella mobilis should be transferred from the genus Zhangella to the genus Maritalea, with the name Maritalea mobilis comb. nov. (type strain E6(T)=CGMCC 1.7002(T)=JCM 15144(T)).International Journal of Systematic and Evolutionary Microbiology 02/2011; 62(Pt 1):43-8. DOI:10.1099/ijs.0.028704-0 · 2.80 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: The taxonomic relationship between Nocardioides salarius CL-Z59(T) and Nocardioides basaltis J112(T) was established. The 16S rRNA gene sequences of the type strains showed 99.6 % similarity. The ΔT(m) for genomic DNA-DNA hybridization of N. salarius CL-Z59(T) and N. basaltis J112(T) was 0.6-1.6 °C, indicating that both strains belong to a single species. Phenotypic and chemotaxonomic characteristics showed no pronounced differences between the two species. Based on the results of the polyphasic approach, it is proposed that N. basaltis J112(T) is a later heterotypic synonym of N. salarius CL-Z59(T). An emended description of the species N. salarius is given.Antonie van Leeuwenhoek 07/2012; 102(4):695-701. DOI:10.1007/s10482-012-9768-1 · 2.14 Impact Factor
Chapter: The Prokaryotes[Show abstract] [Hide abstract]
ABSTRACT: The family Hyphomicrobiaceae, first proposed in 1950, is affiliated with the Alphaproteobacteria. Currently (June 2012) it encompasses 18 genera: Hyphomicrobium [type genus], Ancalomicrobium, Angulomicrobium, Aquabacter, Blastochloris, Cucumibacter, Devosia, Dichotomicrobium, Filomicrobium, Maritalea, Methylorhabdus, Pedomicrobium, Pelagibacterium, Prosthecomicrobium, Rhodomicrobium, Rhodoplanes, Seliberia, and Vasilyevaea, with a total of 54 species. Morphologically and physiologically the family is highly diverse. Many representatives are characterized by the presence of prosthecae, and many species divide by budding. Many prosthecate species are oligocarbophilic, thriving only in the presence of low concentrations of suitable carbon sources and unable to grow in rich media. Most are aerobic chemoheterotrophs. A few representatives can grow anaerobically by denitrification or mixed-acid fermentation. Blastochloris, Rhodomicrobium, and Rhodoplanes are genera of facultative photoheterotrophs. Facultative chemolithotrophy with hydrogen and/or reduced sulfur compounds is also encountered. Representatives of the family can be found worldwide in soils, freshwater lakes and streams, and also in the marine environment.The Prokaryotes, 01/2014: pages 247-281; , ISBN: 978-3-642-30196-4