Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurbits, and legumes. Virology

Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan.
Virology (Impact Factor: 3.32). 03/2009; 386(2):407-16. DOI: 10.1016/j.virol.2009.01.039
Source: PubMed


Apple latent spherical virus (ALSV) vectors were evaluated for virus-induced gene silencing (VIGS) of endogenous genes among a broad range of plant species. ALSV vectors carrying partial sequences of a subunit of magnesium chelatase (SU) and phytoene desaturase (PDS) genes induced highly uniform knockout phenotypes typical of SU and PDS inhibition on model plants such as tobacco and Arabidopsis thaliana, and economically important crops such as tomato, legume, and cucurbit species. The silencing phenotypes persisted throughout plant growth in these plants. In addition, ALSV vectors could be successfully used to silence a meristem gene, proliferating cell nuclear antigen and disease resistant N gene in tobacco and RCY1 gene in A. thaliana. As ALSV infects most host plants symptomlessly and effectively induces stable VIGS for long periods, the ALSV vector is a valuable tool to determine the functions of interested genes among a broad range of plant species.

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    • "Some changes in protein levels would precede the transcriptomic changes, for example, the physical interaction of some host proteins with Tav, the degradation of Tav or some host proteins or both, and post-translational modifications such as phosphorylation, ubiquitination, or nitrosylation of Tav or some host proteins or both. In addition to such analytical studies, the present system is also useful for testing gene function using virus-induced gene silencing (VIGS), because VIGS in tobacco plants is possible using the Apple latent spherical virus vector [38]. Furthermore, the experimental system would also be useful for analyzing the process of chloroplast damage, because the chlorophyll decrease was not rapid and therefore, chloroplasts at different stages of damage would be available using the system. "
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    Physiological and Molecular Plant Pathology 10/2014; 88. DOI:10.1016/j.pmpp.2014.08.005 · 1.41 Impact Factor
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    • "Because most vectors have limited host ranges, vector-mediated HGT from Brassicaceae (or from Fabaceae, in the case of albumin 1[35]) to Orobanchaceae and Convolvulaceae appears to be unlikely. Only vectors with a wide host range, such as apple latent spherical virus [57], may be able to mediate these transfer events. However, no plant viruses carrying the foreign genes have been discovered so far. "
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    BMC Plant Biology 01/2014; 14(1):19. DOI:10.1186/1471-2229-14-19 · 3.81 Impact Factor
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    • "Our finding also implies that many other available viral vectors, such as Apple latent spherical virus (Igarashi et al., 2009; Yamagishi et al., 2011), Brome mosaic virus (Ding et al., 2006), and Barley stripe mosaic virus (Holzberg et al., 2002), could be used in a similar strategy to the TRV VbMS vector for functional analysis of miRNAs in a diverse range of eudicot and monocot crops. In addition, besides IPS1-based miRNA target mimicry and STTM methods, several other techniques, such as transcriptional gene silencing of miRNA gene promoters (Vaistij et al., 2010), artificial miRNA-directed silencing of miRNA precursors (Eamens et al., 2011), and miRNA decoy (Ivashuta et al., 2011), have successfully been used to investigate miRNA/target interactions in transgenic plants. "
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