Article

Co-Regulated Transcriptional Networks Contribute to Natural Genetic Contribute Variation in Drosophila Sleep

Department of Genetics, North Carolina State University, Raleigh, North Carolina 27695, USA.
Nature Genetics (Impact Factor: 29.65). 04/2009; 41(3):371-5. DOI: 10.1038/ng.330
Source: PubMed

ABSTRACT Sleep disorders are common in humans, and sleep loss increases the risk of obesity and diabetes. Studies in Drosophila have revealed molecular pathways and neural tissues regulating sleep; however, genes that maintain genetic variation for sleep in natural populations are unknown. Here, we characterized sleep in 40 wild-derived Drosophila lines and observed abundant genetic variation in sleep architecture. We associated sleep with genome-wide variation in gene expression to identify candidate genes. We independently confirmed that molecular polymorphisms in Catsup (Catecholamines up) are associated with variation in sleep and that P-element mutations in four candidate genes affect sleep and gene expression. Transcripts associated with sleep grouped into biologically plausible genetically correlated transcriptional modules. We confirmed co-regulated gene expression using P-element mutants. Quantitative genetic analysis of natural phenotypic variation is an efficient method for revealing candidate genes and pathways.

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