Monitoring of cell cultures in microbioreactors is a crucial task in cell bioassays and toxicological tests. In this work a novel tool based on a miniaturized sensor array fabricated using low-temperature cofired ceramics (LTCC) technology is presented. The developed device is applied to the monitoring of cell-culture media change, detection of the growth of various species, and in toxicological studies performed with the use of cells. Noninvasive monitoring performed with the LTCC microelectrode array can be applied for future cell-engineering purposes.
"Several applications of LTCC based microfluidic systems such as micro reactors for PCR applications , cell culture analysis systems , environmental  and biosensors  have been reported in literature recently. "
[Show abstract][Hide abstract] ABSTRACT: This paper presents a fully automated low temperature co-fired ceramic (LTCC) based microfluidic system with an integrated electrochemical biosensing platform for the detection of cortisol. This paper presents the design, fabrication, integration and testing of the integrated 3D microfluidic system. The electrochemical immunosensor consists of microfabricated interdigitated Au electrodes, onto which cortisol antibodies are immobilized using a self-assembled monolayer (SAM) matrix of dithiobis(succinimidyl propionate) (DTSP). Finite element based simulation was used to optimize the fluid flow dynamics and washing efficiency required for immunosensing in the LTCC microfluidic assay chamber. Cortisol was used as a model analyte to demonstrate electrochemical immunosensing in a fully automated microfluidic system. Cortisol was detected in a linear range of 10 pM–100 nM at a sensitivity of 0.207 μA/M using cyclic voltammetry (CV). This system establishes the basis for the development of a POC cortisol sensor.
"Monitoring of cell cultures in microbioreactors is a crucial task in cell bioassays and toxicological tests. For such purpose we proposed a miniaturized sensor array fabricated using LTCC technology . The developed device was applied to the monitoring of cell-culture media change, detection of the growth of various species, and in toxicological studies performed with the use of cells . "
[Show abstract][Hide abstract] ABSTRACT: Potentiometric sensors are attractive tools for the fabrication of various electronic tongues that can be used in wide area of applications, ranging from foodstuff recognition to environmental monitoring and medical diagnostics. Their main advantages are the ability to modify their selectivity (including cross-sensitivity effects) and the possibility of miniaturization using appropriate construction methods for the transducer part (e.g., with the use of solid-state technology). In this overview various examples of the design, performance, and applications of potentiometric electronic tongues are presented. The results summarize recent research in the field conducted in the Department of Microbioanalytics, Warsaw University of Technology (WUT).
[Show abstract][Hide abstract] ABSTRACT: In this contribution, a novel measurement approach for miniaturized capillary electrophoresis (CE) devices is presented: End-to-end differential capacitively coupled contactless conductivity measurement. This measurement technique is applied to a miniaturized CE device fabricated in low-temperature cofired ceramics (LTCC) multilayer technology. The working principle is based on the placement of two distinct detector areas near both ends of the fluid inlet and outlet of the separation channel. Both output signals are subtracted from each other, and the resulting differential signal is amplified and measured. This measurement approach has several advantages over established, single-end detectors: The high baseline level resulting from parasitic stray capacitance and buffer conductivity is reduced, leading to better signal-to-noise ratio and hence higher measurement sensitivity. Furthermore, temperature and, thus, baseline drift effects are diminished owing to the differentiating nature of the system. By comparing the peak widths measured with both detectors, valuable information about zone dispersion effects arising during the separation is obtained. Additionally, the novel measurement scheme allows the determination of dispersion effects that occur at the time of sample injection. Optical means of dispersion evaluation are ineffective because of the opaque LTCC substrate. Electrophoretic separation experiments of inorganic ions show sensitivity enhancements by about a factor of 30-60 compared to the single-end measurement scheme.
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