Monitoring of cell cultures with LTCC microelectrode array.
ABSTRACT Monitoring of cell cultures in microbioreactors is a crucial task in cell bioassays and toxicological tests. In this work a novel tool based on a miniaturized sensor array fabricated using low-temperature cofired ceramics (LTCC) technology is presented. The developed device is applied to the monitoring of cell-culture media change, detection of the growth of various species, and in toxicological studies performed with the use of cells. Noninvasive monitoring performed with the LTCC microelectrode array can be applied for future cell-engineering purposes.
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ABSTRACT: Adipose tissue contains a large portion of stem cells. These cells appear morphologically like fibroblasts and are primarily derived from the stromal cell fraction. Mature (lipid-filled) adipocytes possess the ability to become proliferative cells and have been shown to produce progeny cells that possess the same morphological (fibroblast-like) appearance as the stem cells from the stromal fraction. A closer examination of mature adipocyte-derived progeny cells may prove to be an emerging area of growth/metabolic physiology that may modify present thinking about adipose tissue renewal capabilities. Knowledge of these cells may also prove beneficial in cell-based therapies for tissue repair, regeneration, or engineering.Biochemical and Biophysical Research Communications 05/2008; 368(3):455-7. · 2.41 Impact Factor
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ABSTRACT: The emergence of tissue engineering raises new possibilities for the study of complex physiological and pathophysiological processes in vitro. Many tools are now available to create 3D tissue models in vitro, but the blueprints for what to make have been slower to arrive. We discuss here some of the 'design principles' for recreating the interwoven set of biochemical and mechanical cues in the cellular microenvironment, and the methods for implementing them. We emphasize applications that involve epithelial tissues for which 3D models could explain mechanisms of disease or aid in drug development.Nature Reviews Molecular Cell Biology 04/2006; 7(3):211-24. · 37.16 Impact Factor
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ABSTRACT: Integration of various chemical devices and complex operations onto a microchip, which is often referred to as a micro total analysis system (mu-TAS) or lab-on-a-chip, creates extremely efficient devices that exploit the advantages of a microspace. Furthermore, as the scale of the fluidic microvolume is roughly proportional to living cell sizes and processing capabilities, cells and micro chemical systems can be combined to develop practical prototypical microdevices. This approach has led to development of tools for investigating cellular functions, biochemical reactors and bioassay systems, as well as hybrid bio/artificial tissue engineered organs. Recently, bio-microactuators exploiting mechanical properties of cells powered without external energy sources have also been reported. This review focuses on new technologies involving cell-based devices on microchips, with a special emphasis on bio-microactuators. Firstly, we review systems to place and handle cells on a microchip. Secondly, we review bio-microactuators developed using single or a few driving cells. Finally, we review bio-microactuators developed using numerous cells or tissue to generate stronger forces. Understanding fundamental concepts behind the distinct features and performance characteristics of these cell-based micro-systems will lead to development of new devices that will be exploited in various fields in the future.Biosensors and Bioelectronics 12/2007; 23(4):449-58. · 5.44 Impact Factor