Mycobacterium tuberculosis glycoproteomics based on ConA-lectin affinity capture of mannosylated proteins.

Departamento de Inmunologia, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico.
Journal of Proteome Research (Impact Factor: 5.06). 03/2009; 8(2):721-33. DOI: 10.1021/pr800756a
Source: PubMed

ABSTRACT A Mycobacterium tuberculosis culture filtrate enriched with mannose-containing proteins was resolved by 2-DE gel. After ConA ligand blotting, 41 proteins were identified by mass spectrometry as putative glycoproteins with 34 of them new probably mannosylated proteins. These results contribute to the construction of the ConA affinity glycoprotein database of M. tuberculosis, and provide useful information for understanding the biological role of glycoproteins in mycobacteria.

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