Article

Crystallization and preliminary crystallographic characterization of the extrinsic PsbP protein of photosystem II from Spinacia oleracea.

Institute of Systems Biology and Ecology, Academy of Sciences of Czech Republic, Zámek 136, 37333 Nové Hrady, Czech Republic.
Acta Crystallographica Section F Structural Biology and Crystallization Communications (impact factor: 0.51). 03/2009; 65(Pt 2):111-5. DOI:10.1107/S1744309108040578 pp.111-5
Source: PubMed

ABSTRACT Preliminary X-ray diffraction analysis of the extrinsic PsbP protein of photosystem II from spinach (Spinacia oleracea) was performed using N-terminally His-tagged recombinant PsbP protein overexpressed in Escherichia coli. Recombinant PsbP protein (thrombin-digested recombinant His-tagged PsbP) stored in bis-Tris buffer pH 6.00 was crystallized using the sitting-drop vapour-diffusion technique with PEG 550 MME as a precipitant and zinc sulfate as an additive. SDS-PAGE analysis of a dissolved crystal showed that the crystals did not contain the degradation products of recombinant PsbP protein. PsbP crystals diffracted to 2.06 A resolution in space group P2(1)2(1)2(1), with unit-cell parameters a = 38.68, b = 46.73, c = 88.9 A.

0 0
 · 
0 Bookmarks
 · 
35 Views
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

bis-Tris buffer pH 6.00
 
degradation products
 
N-terminally His-tagged recombinant PsbP protein overexpressed
 
precipitant
 
Preliminary X-ray diffraction analysis
 
recombinant PsbP protein
 
sitting-drop vapour-diffusion technique
 
spinach
 
thrombin-digested recombinant His-tagged PsbP
 
unit-cell parameters
 
zinc sulfate