Radioprotective effects of hesperidin against genotoxicity induced by -irradiation in human lymphocytes

Department of Medicinal Chemistry, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Mutagenesis (Impact Factor: 2.79). 03/2009; 24(3):233-5. DOI: 10.1093/mutage/gep001
Source: PubMed


The radioprotective effect of hesperidin against genotoxicity induced by gamma-irradiation has been investigated in vivo/in vitro in cultured blood lymphocytes from human volunteers. Peripheral blood samples were collected at 0 (10 min before) and at 1, 2 and 3 h after a single oral ingestion of 250 mg hesperidin. At each time point, the whole blood was exposed in vitro to 150 cGy of (60)Co gamma-irradiation and then the lymphocytes were cultured with mitogenic stimulation to determine the micronuclei in cytokinesis-blocked binucleated cells. For each volunteer, the results showed a significant increase in the incidence of micronuclei after exposure of cells to gamma-irradiation as compared to control samples. The lymphocytes in the blood samples collected at 1 h after hesperidin ingestion and exposed in vitro to gamma-rays exhibited a significant decrease in the incidence of micronuclei, compared with similarly irradiated lymphocytes from blood samples collected at 0 h. The maximum protection and decrease in frequency of micronuclei (33%) was observed at 1 h after ingestion of hesperidin. These data have important application for the protection of human lymphocytes from the genetic damage and side effects induced by gamma-irradiation in patients undergoing radiotherapy.

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    • "The antioxidant property of these flavonoids was determined by the DPPH assay and showed significant antioxidant properties [35]. We previously reported that medicinal plants and natural products such as flavonoids and phenolic compounds with antioxidant properties and free radical-scavenging mechanism reduced genotoxicity and micronucleus formation in human blood lymphocytes when administrated prior to genotoxic agents [39–42]. We recently reported that O. vulgare pretreatment attenuated radiation-induced oxidative stress and the subsequent DNA damage in human blood lymphocyte. "
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    • "In conclusion, we have provided evidence that hesperidin inhibits TPA-induced cell invasiveness and MMP-9 expression in human cancer cells by suppressing both the AP-1 and NF-kB signaling pathways (Fig. 8). Hesperidin also has in vitro radioprotective and chemoprotective effects (Hosseinimehr et al., 2009; Kalpana et al., 2009; Aranganathan et al., 2009) and is an effective supplemental agent in complementary settings (Tirkey et al., 2005). Such findings suggest that hesperidin could be of therapeutic value in preventing invasion or metastasis of human cancers. "
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