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Stem cells and tissue-engineered skin

Department of Dermatology, University of California and Veterans Affairs Medical Center, San Francisco, CA, USA.
Skin pharmacology and physiology (Impact Factor: 1.96). 02/2009; 22(2):55-62. DOI: 10.1159/000178864
Source: PubMed

ABSTRACT Advances in tissue engineering of skin are needed for clinical applications (as in wound healing and gene therapy) for cutaneous and systemic diseases. In this paper we review the use of epidermal stem cells as a source of cells to improve tissue-engineered skin. We discuss the importance and limitations of epidermal stem cell isolation using biomarkers, in quest of a pure stem cell preparation, as well as the culture conditions necessary to maintain this purity as required for a qualitatively superior and long-lasting engineered skin. Finally, we review the advantages of using additional multipotent stem cell sources to functionally and cosmetically optimize the engineered tissue.

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Available from: Ruby Ghadially, May 26, 2015
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    • "Therefore, genetically modified, in vitro-expanded adult stem cells are attractive alternatives for use in gene therapy. In this regard, one previous study demonstrated the formation of clonal units of epidermal structures after infusion of mouse ESCs transduced with retroviruses (Charruyer and Ghadially, 2009). The cell cycle mainly proceeds in three phases, the G0/G1 phase, S phase, and G2/M phase. "
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    • "Epidermal stem cells are located in the basal layer of the epidermis and can differentiate into keratinocytes [Blanpain and Fuchs, 2009; Charruyer and Ghadially, 2009]. However, there are relatively few data on the effects of EMF on epidermal stem cells, which are regarded as the progenitor cells of keratinocytes and defined by b1-integrin (known as CD29) þ /CD71 À expression [Barthel and Aberdam, 2005]. "
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