Identification of CD15 as a Marker for Tumor-Propagating Cells in a Mouse Model of Medulloblastoma

Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA.
Cancer cell (Impact Factor: 23.52). 03/2009; 15(2):135-47. DOI: 10.1016/j.ccr.2008.12.016
Source: PubMed


The growth of many cancers depends on self-renewing cells called cancer stem cells or tumor-propagating cells (TPCs). In human brain tumors, cells expressing the stem cell marker CD133 have been implicated as TPCs. Here we show that tumors from a model of medulloblastoma, the Patched mutant mouse, are propagated not by CD133(+) cells but by cells expressing the progenitor markers Math1 and CD15/SSEA-1. These cells have a distinct expression profile that suggests increased proliferative capacity and decreased tendency to undergo apoptosis and differentiation. CD15 is also found in a subset of human medulloblastomas, and tumors expressing genes similar to those found in murine CD15(+) cells have a poorer prognosis. Thus, CD15 may represent an important marker for TPCs in medulloblastoma.

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    • "Approximately 17.6% (3 out of 17 PtenFloxp/+) of mice expressing one allele of Ptch1 developed symptoms of medulloblastoma by 1 year of age. Most of these mice died 5 months after birth[22],[23]. However, PtenFloxp/+ PtenFloxp/+ GFAP-Cre mice died of medulloblastoma from 2 months after birth. "
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    • "It has been reported recently, that CD133 is only expressed in a subset of glioblastomas [43], while observation of abundant nestin expression is in complete agreement with several previous reports that came to the same conclusion [35]–[37]. Furthermore, specific CD15 immunoreactivity on BTSCs has been described by several groups [38], [39], [44], [45]. We did observe a small number of apparent CD15+ cells that were judged to be astrocytic glioma cells, based on size. "
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