Article

Oligomeric structure of LOV domains in Arabidopsis phototropin.

Department of Biological Science, Graduate School of Science, Osaka Prefecture University, Naka-ku, Sakai, Osaka, Japan.
FEBS letters (impact factor: 3.54). 02/2009; 583(3):526-30. DOI:10.1016/j.febslet.2009.01.019 pp.526-30
Source: PubMed

ABSTRACT Oligomeric structures of the four LOV domains in Arabidopsis phototropin1 (phot1) and 2 (phot2) were studied using crosslinking. Both LOV1 domains of phot1 and phot2 form a dimer independently on the light conditions, suggesting that the LOV1 domain can be a stable dimerization site of phot in vivo. In contrast, phot1-LOV2 is in a monomer-dimer equilibrium and phot2-LOV2 exists as a monomer in the dark. Blue light-induced a slight increase in the monomer population in phot1-LOV2, suggesting a possible blue light-inducible dissociation of dimers. Furthermore, blue light caused a band shift of the phot2-LOV2 monomer. CD spectra revealed the unfolding of helices and the formation of strand structures. Both light-induced changes were reversible in the dark.

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Keywords

Arabidopsis phototropin1
 
blue light
 
CD spectra
 
four LOV domains
 
light conditions
 
light-induced changes
 
LOV1 domain
 
LOV1 domains
 
monomer-dimer equilibrium
 
Oligomeric structures
 
phot1-LOV2
 
phot2
 
phot2 form
 
phot2-LOV2
 
phot2-LOV2 monomer
 
possible blue light-inducible dissociation
 
slight increase
 
stable dimerization site
 
vivo
 

Hitomi Katsura