Article

Global map of SUMO function revealed by protein-protein interaction and genetic networks.

Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON M5S3E1, Canada.
Molecular cell (impact factor: 14.61). 02/2009; 33(1):124-35. DOI:10.1016/j.molcel.2008.12.025 pp.124-35
Source: PubMed

ABSTRACT Systematic functional genomics approaches were used to map a network centered on the small ubiquitin-related modifier (SUMO) system. Over 250 physical interactions were identified using the SUMO protein as bait in affinity purification-mass spectrometry and yeast two-hybrid screens. More than 500 genes and 1400 synthetic genetic interactions were mapped by synthetic genetic array (SGA) analysis using eight different SUMO pathway query genes. The resultant global SUMO network highlights its role in 15 major biological processes and better defines functional relationships between the different components of the SUMO pathway. Using this information-rich resource, we have identified roles for the SUMO system in the function of the AAA ATPase Cdc48p, the regulation of lipid metabolism, localization of the ATP-dependent endonuclease Dna2p, and recovery from the DNA-damage checkpoint.

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Keywords

1400 synthetic genetic interactions
 
15 major biological processes
 
250 physical interactions
 
AAA ATPase Cdc48p
 
defines functional relationships
 
different components
 
different SUMO pathway query genes
 
DNA-damage checkpoint
 
resultant global SUMO network
 
roles
 
small ubiquitin-related modifier
 
SUMO system
 
synthetic genetic array
 
Systematic functional genomics approaches