A novel technique for NACE coupled with simultaneous electrochemiluminescence and electrochemical detection for fast analysis of tertiary amines.

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, P. R. China.
Electrophoresis (Impact Factor: 3.26). 02/2009; 30(3):479-86. DOI: 10.1002/elps.200800253
Source: PubMed

ABSTRACT A simultaneous electrochemiluminescence (ECL) and electrochemical (EC) detection scheme for NACE was presented for fast analysis of tertiary amines. Both ECL and EC signals were generated at the same Pt electrode. Triethylamine (TEA), tripropylamine (TPrA), chlorpromazine, promethazine, and dioxopromethazine (DPZ) were selected to validate NACE-ECL/EC dual detection strategy. The linear ranges for TEA and TPrA were 0.01-500 and 0.01-10 microM with the detection limits of 8.0 and 5.0 nM (S/N=3), respectively. The RSDs (n=6) of the migration time and the ECL intensity for 1 microM TEA and 0.5 microM TPrA were 0.1 and 2.8%, and 0.2 and 1.8% with theoretical plate numbers of 180,000 and 700,000 per meter, respectively. These two analytes could be separated within 92 s and the Pt electrode did not need reactivation during the experiments. Chlorpromazine, promethazine, and DPZ could be well separated by NACE. The proposed method was also demonstrated for fast determination of DPZ in human urine with simple sample preparation. The results indicated that NACE-ECL/EC had the advantages of simple and fast analysis with more information, wide linear range, high sensitivity, and compatibility with real urine sample.

  • [Show abstract] [Hide abstract]
    ABSTRACT: Analyses of very complex samples involving capillary and chip electrophoresis often require dual (multiple) detection to attain sufficient selectivity of determination. The present work reviews and critically evaluates selected combinations of electrochemical detection techniques among themselves and with absorption spectrometric, fluorescence and luminescence techniques. Amperometry, contact and contactless conductometry, UV photometry and fluorescence measurements are paid special attention. Some information is also given on combinations of spectrometric techniques with mass spectrometry. The properties of the detection systems are critically discussed, examples are illustrated in figures and some details on the characteristics of dual detectors and their applications are tabulated.
    Electrophoresis 03/2011; 32(8):795-810. · 3.26 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Electrochemiluminescence (ECL) is chemiluminescence triggered by electrochemical techniques. More than 150 ECL assays with remarkably high sensitivity and extremely wide dynamic range are currently available, and accounts for hundreds of millions of dollars in sales per year. The recent development of ECL is particularly rapid. After a brief introduction to ECL, this critical review presents the active and/or emerging areas of ECL research as well as new applications and phenomena of ECL, such as light-emitting electrochemical cell, wireless electrochemical microarray using ECL as photonic reporter, high throughput analysis, aptasensors, immunoassays and DNA analysis, ECL of nanoclusters and carbon nanomaterials, ECL imaging techniques, scanning ECL microscopy, colorimetric ECL sensor, surface plasmon-coupled ECL, electrostatic chemiluminescence, soliton-like ECL waves, ECL investigation of molecular interaction, and single molecule detection. Finally, some perspectives on this rapidly developing field are discussed (322 references).
    Chemical Society Reviews 08/2010; 39(8):3275-304. · 24.89 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A sensitive approach for the simultaneous determination of tilmicosin, erythromycin ethylsuccinate and clindamycin was developed by CE coupled with electrochemiluminescence detection with ionic liquid. The parameters for CE, electrochemiluminescence detection and the effect of ionic liquid were investigated systematically. The three analytes were well separated and detected within 8 min. The limits of detection (S/N=3) of tilmicosin, erythromycin ethylsuccinate and clindamycin are 3.4x10(-9), 2.3x10(-8) and 1.3x10(-8) mol/L, respectively. The precisions (RSD%) of the peak area and the migration time are from 0.8 to 1.5% and from 0.2 to 0.5% within a day and from 1.8 to 2.7% and from 0.6 to 0.8% in 3 days, respectively. The limits of quantitation (S/N=10) of tilmicosin, erythromycin ethylsuccinate and clindamycin are 3.2x10(-8), 2.9x10(-7) and 9.1x10(-8) mol/L in human urines and 5.5x10(-8), 3.2x10(-7) and 2.1x10(-7) mol/L in milk samples, respectively. The recoveries of three analytes at different concentration levels in urine, milk and drugs are between 90.0 and 104.7%. The proposed method was successfully applied to the determination of three analytes in human urine, milk and drugs.
    Journal of Separation Science 03/2010; 33(9):1305-11. · 2.59 Impact Factor