Detection of Illicium anisatum as Adulterant of Illicium verum

National Center for Natural Products Research and Research Institute of Pharmaceutical Sciences, Department of Pharmacognosy, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
Planta Medica (Impact Factor: 2.15). 02/2009; 75(4):392-5. DOI: 10.1055/s-0028-1112219
Source: PubMed


Chinese Star anise, Illicium verum Hook, is a well known spice in many cultures and has also been used to treat infant colic. Recent publications report that Chinese Star anise might be adulterated with the toxic Japanese Star anise, Illicium anisatum L. We have developed a molecular method that helps with the detection of I. anisatum as adulterant of I. verum. We PCR-amplified the internal transcribed spacer (ITS) region and analyzed it with the endonucleases PSTI and BFMI. Based on fragment length polymorphisms (RFLP), we were able to detect and distinguish between I. anisatum and other Illicium species in powdered samples.

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Available from: Natascha Techen, Oct 13, 2015
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    • ". Fluorescence microscopy, thermal desorption–gas chromatography–mass spectrometry, thin layer chromatography (TLC), high performance liquid chromatography coupled to mass spectrometry (HPLC-MS/MS), DNA sequencing and restriction fragment length polymorphism (RFLP) methods have all been proposed with varying levels of success [10] [14] [16] [18] "
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