Article

Photoacoustic Microscopy in Tissue Engineering.

Department of Biomedical Engineering, Washington University, St. Louis, Missouri 63130, USA.
Materials Today (Impact Factor: 10.85). 03/2013; 16(3):67-77. DOI: 10.1016/j.mattod.2013.03.007
Source: PubMed

ABSTRACT Photoacoustic tomography (PAT) is an attractive modality for noninvasive, volumetric imaging of scattering media such as biological tissues. By choosing the ultrasonic detection frequency, PAT enables scalable spatial resolution with desired imaging depth up to ~7 cm while maintaining a high depth-to-resolution ratio of ~200 and consistent optical absorption contrasts. Photoacoustic microscopy (PAM), the microscopic embodiment of PAT, aims to image at millimeter depth and micrometer-scale resolution. PAM is well-suited for characterizing three-dimensional scaffold-based samples, including scaffolds themselves, cells, and blood vessels, both qualitatively and quantitatively. Here we review our previous work on applications of PAM in tissue engineering and then discuss its future developments.

1 Follower
 · 
72 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: Optical-resolution photoacoustic microscopy (OR-PAM) is an imaging modality with superb penetration depth and excellent absorption contrast. Here we demonstrate, for the first time, that this technique can advance quantitative analysis of conventional chromogenic histochemistry. Because OR-PAM can quantify the absorption contrast at different wavelengths, it is feasible to spectrally resolve the specific biomolecules involved in a staining color. Furthermore, the tomographic capability of OR-PAM allows for noninvasive volumetric imaging of a thick sample without microtoming it. By immunostaining the sample with different chromogenic agents, we further demonstrated the ability of OR-PAM to resolve different types of cells in a coculture sample with imaging depths up to 1 mm. Taken together, the integration of OR-PAM with (immuno)histochemistry offers a simple and versatile technique with broad applications in cell biology, pathology, tissue engineering, and related biomedical studies.
    Angewandte Chemie International Edition in English 07/2014; 53(31). DOI:10.1002/anie.201403812 · 13.45 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Adequate vascularisation is key in determining the clinical outcome of stem cells and engineered tissue in regenerative medicine. Numerous imaging modalities have been developed and used for the visualization of vascularisation in tissue engineering. In this review, we briefly discuss the very recent advances aiming at high performance imaging of vasculature. We classify the vascular imaging modalities into three major groups: nonoptical methods (X-ray, magnetic resonance, ultrasound, and positron emission imaging), optical methods (optical coherence, fluorescence, multiphoton, and laser speckle imaging), and hybrid methods (photoacoustic imaging). We then summarize the strengths and challenges of these methods for preclinical and clinical applications.
    BioMed Research International 01/2015; 783983. DOI:10.1155/2015/783983 · 2.71 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Tissue engineering has evolved with multifaceted research being conducted using advanced technologies, and it is progressing towards clinical applications. As tissue engineering technology significantly advances, it proceeds towards increasing sophistication, including nanoscale strategies for material construction and synergetic methods for combining with cells, growth factors, or other macromolecules. Therefore, in order to assess advanced tissue engineered constructs, tissue engineers need versatile imaging methods capable of monitoring not only morphological but also functional and molecular information. However, there is no single imaging modality that is suitable for all tissue engineered constructs. Each imaging method has its own range of applications and provides information based on the specific properties of the imaging technique. Therefore, according to the requirements of the tissue engineering studies, the most appropriate tool should be selected among a variety of imaging modalities. The goal of this review paper is to describe available biomedical imaging methods to assess tissue engineering applications and to provide tissue engineers with criteria and insights for determining the best imaging strategies. Commonly used biomedical imaging modalities, including X-ray and computed tomography (CT), positron emission tomography (PET) and single photon emission computed tomography (SPECT), magnetic resonance imaging (MRI), ultrasound imaging, optical imaging, and emerging techniques and multimodal imaging, will be discussed, focusing on the latest trends of their applications in recent tissue engineering studies.
    Tissue Engineering Part B Reviews 07/2014; DOI:10.1089/ten.TEB.2014.0180 · 4.64 Impact Factor

Preview

Download
4 Downloads
Available from