Article

Genetic polymorphisms of mannose-binding lectin do not influence placental malaria but are associated with preterm deliveries.

Department of Biology, Georgetown University, Washington, DC, USA.
Infection and immunity (impact factor: 4.21). 02/2009; 77(4):1483-91. DOI:10.1128/IAI.01069-08
Source: PubMed

ABSTRACT During pregnancy, Plasmodium falciparum-infected erythrocytes (IE) sequester in the placenta where they induce pathology and increase the risk of low-birth-weight (LBW) babies. The innate immune mediator, mannose-binding lectin (MBL), enhances phagocytosis of pathogens. Since MBL is reported to bind to IE, we hypothesized that it might aid in clearance of IE from the placenta, thereby reducing the risk of LBW babies. To test this hypothesis, molecular genotyping was used to detect polymorphisms at codon 57 (A/C) in exon 1 of MBL2 in 401 pregnant Cameroonian women, with or without placental malaria, who had LBW and normal-weight babies. Polymorphisms in the promoter region at positions -550 (H/L), -221 (X/Y), and +4 (P/Q) were also determined, and plasma MBL levels were measured during pregnancy and at delivery. The expected correlation between genotype and plasma MBL levels was confirmed. However, asymptomatic infections were not associated with an increase in MBL levels in the peripheral blood, and MBL levels were similar in the placental and cord blood of women with or without placental malaria at delivery. There was no evidence that MBL levels at delivery were associated with malaria-related poor pregnancy outcomes. Women with the LXPA haplotype, however, were more likely to have LBW babies, but the risk was not related to malaria. These results do not support the hypothesis that MBL aids in the clearance of parasites from the placenta but suggest that Cameroonian women with LXPA are at risk of having LBW babies due to other causes.

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Keywords

401 pregnant Cameroonian women
 
asymptomatic infections
 
Cameroonian women
 
enhances phagocytosis
 
exon 1
 
expected correlation
 
innate immune mediator
 
LBW babies
 
malaria-related poor pregnancy outcomes
 
mannose-binding lectin
 
MBL2
 
molecular genotyping
 
normal-weight babies
 
placental
 
placental malaria
 
plasma MBL levels
 
Plasmodium falciparum-infected erythrocytes
 
Polymorphisms
 
positions -550
 
promoter region