Article

Immunization with Bin1b decreases sperm motility with compromised fertility in rats.

Epithelial Cell Biology Research Center, Li Ka Shing Institute of Health Sciences, Department of Physiology, Key Laboratory for Regenerative Medicine of Ministry of Education, Chinese University of Hong Kong, Hong Kong, People's Republic of China.
Fertility and sterility (impact factor: 3.97). 02/2009; 93(3):952-958.e1. DOI:10.1016/j.fertnstert.2008.10.066
Source: PubMed

ABSTRACT To evaluate the contraceptive ability of a synthetic Bin1b peptide in vivo in the rat.
Basic research.
University laboratory animal service center.
A peptide-based immunization model was developed; rats were injected with the Bin1b specific peptide.
A synthetic peptide segment, MCRSGERKGDICSDP-conjugated with KLH (Bin1b), was used to immunize male wistar rats. Freund's complete adjuvant was used as a control.
Anti-Bin1b levels in sera were evaluated by enzyme-linked immunosorbent assay (ELISA). Anti-Bin1b and control antisera were used to evaluate sperm function inhibition in vitro. The fertility of immunized rats was determined by mating experiment. The testis and epididymides were analyzed by histology.
Histological studies showed no evidence of orchitis or epididymitis in Bin1b-immunized animals. ELISA results revealed that the titers of anti-Bin1b antibodies in serum increased with the immunization process in immunized rats. Sperm recovered from the corpus epididymidis of the Bin1b-immunized animals exhibited a significant decrease in motility. Immunization of Bin1b also caused a reduction (25%) in fertility after the mating experiment.
The present study has demonstrated that immunization with Bin1b peptide specifically interferes with sperm motility, resulting in a compromised fertilizing capacity of sperm.

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Keywords

Anti-Bin1b
 
anti-Bin1b antibodies
 
Anti-Bin1b levels
 
Basic research
 
Bin1b specific peptide
 
Bin1b-immunized animals
 
Bin1b-immunized animals exhibited
 
compromised fertilizing capacity
 
control antisera
 
enzyme-linked immunosorbent assay
 
Freund's complete adjuvant
 
Histological studies
 
immunize male wistar rats
 
immunized rats
 
peptide-based immunization model
 
significant decrease
 
sperm function inhibition
 
synthetic Bin1b peptide
 
synthetic peptide segment
 
University laboratory animal service center