Highlights of a new type of intercellular communication: microvesicle-based information transfer. Inflamm Res

Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
Agents and Actions (Impact Factor: 2.35). 02/2009; 58(1):1-8. DOI: 10.1007/s00011-008-8210-7
Source: PubMed


Microvesicles (MVs) are membrane-covered cell fragments released by most cell types during apoptosis or activation. They are increasingly considered to play a pivotal role in information transfer between cells. Their presence and role have been proven in several physiological and pathological processes, such as immune modulation in inflammation and pregnancy, or blood coagulation and cancer. MVs represent a newly recognized system of intercellular communications. They not only may serve as prognostic markers in different diseases, but could also hold the potential to be new therapeutic targets or drug delivery systems. The present overview aims to highlight some aspects of this new means of cellular communication: "microvesicular communication".


Available from: Eva Pállinger, Dec 17, 2013
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    • "In our model, we analyzed MVs that were present in the cellfree supernatants of co-cultured NAD cells and CCl 4 -injured hepatocytes after the differentiation of NAD cells into hepatocytelike cells. The ultracentrifugation method employed in the present study (100,000 Â g for 60 min) is widely accepted for the isolation of MVs larger than 100 nm (Pap et al., 2009). Zetasizer analysis showed that the MVs formed a rather heterogeneous population with sizes ranging from 100 nm to more than 1 μm. "
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    ABSTRACT: The ability of bone marrow-derived mononuclear cells (BMMCs) to differentiate into hepatocyte-like cells under different conditions has been demonstrated previously. In the present study, we investigated the effect of CCl4-injured hepatocytes on the differentiation of the non-adherent (NAD) fraction of BMMCs. Differentiation (cell fate) was analyzed after 2, 6 and 24h of co-culture by gene and protein expression and by urea production. We also evaluated the presence of microvesicles (MVs) in the supernatant of differentiated cells, their content and the ability of these cells to absorb them. Hepatocyte-like characteristics were observed in the NAD cells after 24h of co-culture with injured hepatocytes. Cells that were co-cultured with healthy hepatocytes did not present signs of differentiation at any analyzed time point. Analysis of the supernatant from differentiated cells revealed the presence of MVs carrying hepatocyte-specific mRNAs, including Albumin, Coagulation factor V, Alpha-fetoprotein, and Cytokeratin 18. The incorporation of injured hepatocyte-derived MVs by NAD cells was shown at 24h, suggesting a possible role for MVs in the induction of cell plasticity.
    Differentiation 09/2015; DOI:10.1016/j.diff.2015.09.001 · 3.44 Impact Factor
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    • "Particularly, an increase of circulating NVs originating from blood cells was demonstrated as marker for atherosclerosis exacerbation (Cocucci et al., 2009). Furthermore, NVs used as biomarker for neurodegenerative disorders, diabetes, rheumatic diseases, cancer and other diseases (Pap et al., 2009; Cocucci et al., 2009). "
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    ABSTRACT: Nanovesicles (NVs) represent a novel transporter for cells signals to modify functions of target cells. Therefore, NVs play many roles in both physiological and pathological process. This report highlights biogenesis, composition and biological roles of erythrocytes derived nanovesicles (EDNVs). Furthermore, we address utilization of EDNVs as novel drug delivery cargo as well as therapeutic target. EDNVs are lipid bilayer vesicles rich in phospholipids, proteins, lipid raft, and hemoglobin. In vivo EDNVs biogenesis is triggered by an increase of intracellular calcium levels, ATP depletion and under effect of oxidative stress conditions. However, in vitro production of EDNVs can be achieved via hypotonic treatment and extrusion of erythrocyte. NVs can be used as biomarkers for diagnosis, monitoring of therapy and drug delivery system. Many therapeutic agents are suggested to decrease NVs biogenesis.
    Saudi Pharmaceutical Journal 07/2015; 4. DOI:10.1016/j.jsps.2015.06.010 · 1.28 Impact Factor
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    • "They have been identified in human, rodent, and fetal calf sera [22] [23] [24] [25] [26] [27]. They are released both by the cells of haematopoietic and non-haematopoietic origin [28] [29], quiescent and activated [30], and non-transformed and tumor cells [31]. Because of their involvement in the intercellular signaling, the examination of their protein components in the healthy and diseased individuals may provide valuable markers for determining the site, type, and an extent of injury in various pathological conditions. "
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    ABSTRACT: Extracellular vesicles have created great interest as possible source of biomarkers for different biological processes and diseases. Although the biological function of these vesicles is not fully understood, it is clear that they participate in the removal of unnecessary cellular material and act as carriers of various macromolecules and signals between the cells. In this report, we analyzed the proteome of extracellular vesicles secreted by primary hepatocytes. We used one- and two-dimensional liquid chromatography combined with data-independent mass spectrometry. Employing label-free quantitative proteomics, we detected significant changes in vesicle protein expression levels in this in vitro model after exposure to well-known liver toxins (galactosamine and Escherichia coli-derived lipopolysaccharide). The results allowed us to identify candidate markers for liver injury. We validated a number of these markers in vivo, providing the basis for the development of novel methods to evaluate drug toxicity. This report strongly supports the application of proteomics in the study of extracellular vesicles released by well-controlled in vitro cellular systems. Analysis of such systems should help to identify specific markers for various biological processes and pathological conditions. Identification of low invasive candidate marker for hepatotoxicity. Support to apply proteomics in the study of extracellular vesicles released by well-controlled in vitro cellular systems to identify low invasive markers for diseases.
    Journal of proteomics 04/2014; 103. DOI:10.1016/j.jprot.2014.04.008 · 3.89 Impact Factor
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