Apple peel extracts and their fractions pooled according to their molecular size were prepared and evaluated for their inhibitory activity against Helicobacter pylori and Jack bean ureases. Urease Inhibitory effect of apple peel polyphenols (APPE) extracted from the Granny Smith variety was concentration-dependent and reversible. High molecular weight polyphenols (HMW) were more active against Helicobacter pylori and Jack bean ureases than low molecular weight polyphenols with IC50 values of 119 and 800 microg GAE/mL, respectively. The results suggest that monomeric compounds (mainly flavan-3-ols-and quercetin-O-glycosides) will not be implicated in the antiurease effect displayed by the apple peel polyphenolic extract. Thus, as a byproduct, apple peel is suitable for developing functional ingredients that could be useful for neutralizing an important Helicobacter pylori colonization factor.
"Apple peel polyphenols extracts . Apple peel polyphenols extracts ( APPE ) have been correlated with gastro - protective effects on chemically induced injury ( Graziani et al . , 2005 ) . It was also hypothesized their role in ameliorating chronic gastrointestinal affections induced by H . pylori via urease inhibition ( Pastene et al . , 2009b ) . APPE , like those rich in procyanidins ( oligomeric compounds built from catechin and epicatechin molecules ) ( Figure 7a and b ) , appear to inhibit the adherence of H . pylori to the gastric mucosa , and the urease activity and / or the cytotoxic effects of the VacA protein ( Pastene et al . , 2010 ; Rohdewald & Beil , 2008 ) . Pa"
[Show abstract][Hide abstract] ABSTRACT: Abstract Helicobacter pylori is a human gastric pathogen considered as the etiologic agent of several gastric disorders, that may range from chronic gastritis to more severe outcomes, including gastric cancer. The current therapeutic scheme relies on the combination of several pharmacological substances, namely antibiotics and proton pump inhibitors. However, the cure rates obtained have been declining over the years, mostly due to bacterial resistance to antibiotics. In this context, the use of non-antibiotic substances is of the utmost importance regarding H. pylori eradication. In this review, we present different classes of compounds obtained from natural sources that have shown to present anti-H. pylori potential; we briefly highlight their possible use in the context of developing new therapeutic approaches.
Critical Reviews in Microbiology 03/2014; DOI:10.3109/1040841X.2014.892055 · 6.02 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: form only given. A series of very regular Poly(3-(4-octylphenyl)thiophene)s (POPT), with a varying ratio of monomer to coupling agent (FeCl/sub 3/), and Poly(3-(4-octylphenyl)-2,2'-bithiophene) (POPT/sub 2/) were studied by X-ray diffraction and UV-VIS absorption spectroscopy. The results show that POPT and POPT/sub 2/ are polymers with a low degree of crystallinity, which could be increased by using a more gentle polymerization condition. The diffraction patterns indicate that the polymers, in the amorphous and crystalline phase, pack as relatively straight chains with a long conjugation length as revealed by the absorption spectra. The crystalline structure is formed by planar polymer back-bones stacking on top of each other and the side chains act as spacers between the stacks. This structure resembles closely the one of Poly(3-octylthiophene) (POT), except for the repetition distance along the side chains, which is increased relative to POT by an amount compatible with the space needed for the additional phenyl group. The slight reduction of this repetition distance in POPT/sub 2/ relative to POPT can be accounted for by the increased space available to the side chains.
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