Expansion of CD3/CD16/CD56 positive NKT cells in HIV/AIDS: the pilot study.

Infectious Diseases, AIDS and Clinical Immunology Research Center, Tbilisi.
Georgian medical news 01/2009;
Source: PubMed

ABSTRACT NKT cells are a subset of lymphocytes possessing features of NK cells and T cells; they play a key role in the formation of innate immune response. Upon stimulation, rapid production of large quantities of both T(h1) and T(h2) type cytokines permits them to bridge the innate and adaptive immune responses by activating NK cells, T cells, B cells and dendritic cells. Scientific knowledge has been collecting up to date toward the definition of the role of NKT lymphocytes in HIV/AIDS setting. This direction in HIV/AIDS immunopathogenesis is relatively new and quite concerning. The objective of this study was to investigate CD3+/CD16+/CD56+ NKT cell expansion in HIV/AIDS patients and explore its association with virologic and immunologic markers of HIV infection. Retrospective analysis of 30 HIV infected patients data, taken from the database of the laboratory of clinical immunology at the Infectious Diseases, AIDS and Clinical Immunology Research Center, was conducted. Results: there was slightly increased risk of higher plasma viral load related to lower NKT cell expansion. With regard to immunologic status, borderline significance between expansion of CD3/CD16/CD56 positive NKT cells and lower CD4 positive cell count was shown. However, study did not show strong associations of NKT cell expansion with either virologic or immunologic status, interestingly, all HIV/TB co infected patients where NKT cell positive, which underlines the possible role of TB in CD3+/CD16+/CD56+ NKT cell expansion phenomena in HIV infected individuals. We think these new findings may serve as prerequisite for future, larger scale research in this direction.

  • [Show abstract] [Hide abstract]
    ABSTRACT: In this study, we examined the dynamics of cellular immune responses in the acute phase of dengue virus (DENV) infection in a marmoset model. Here, we found that DENV infection in marmosets greatly induced responses of CD4/CD8 central memory T and NKT cells. Interestingly, the strength of the immune response was greater in animals infected with a dengue fever strain than in those infected with a dengue hemorrhagic fever strain of DENV. In contrast, when animals were re-challenged with the same DENV strain used for primary infection, the neutralizing antibody induced appeared to play a critical role in sterilizing inhibition against viral replication, resulting in strong but delayed responses of CD4/CD8 central memory T and NKT cells. The results in this study may help to better understand the dynamics of cellular and humoral immune responses in the control of DENV infection.
    Archives of Virology 02/2013; DOI:10.1007/s00705-013-1618-6 · 2.28 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to determine whether there was any association between peripheral blood NKT-like cells and IVF treatment outcome. In this prospective study, 124 women who underwent IVF treatment for tubal factor were randomly selected in June and July 2011. Blood samples were obtained on the day of vaginal oocyte retrieval prior to the procedure. The percentages of peripheral blood NKT-like cell subsets and the expression levels of mRNA encoding MCP-1 and TNFα in PBMCs were recorded, and their relation to IVF treatment outcomes was analyzed. CD3+CD56+CD16+ NKT-like cells were significantly elevated in the pregnant group. The expression level of MCP-1 and TNFα mRNAs in PBMCs from the pregnant group was also increased. Furthermore, both MCP-1 and TNFα mRNA expression were positively correlated with the percentage of CD3+CD56+CD16+ NKT-like cells (r=0.55 and r=0.70, respectively). A ROC analysis (AUC=0.634) found that in those women with CD3+CD56+CD16+ NKT-like cells >1.85%, the pregnancy rate and live birth rate were higher than in women with cells below this level. An increase in CD3+CD56+CD16+ NKT-like cells was associated with a better IVF treatment outcome (OR 4.89, 95% CI=1.86-12.88), with a sensitivity of 47%, specificity of 85%, PPV of 89%, and NPV of 56%. We conclude that an elevated level of circulating CD3+CD56+CD16+ NKT-like cells is associated with increased rates of pregnancy and live births in IVF treatment.
    Journal of Reproductive Immunology 02/2013; DOI:10.1016/j.jri.2012.12.006 · 2.37 Impact Factor


Available from
Jun 1, 2014