Hiscox JD, Israelstam GF A method for the extraction of chlorophyll from leaf tissue without maceration. Can J Bot

Canadian Journal of Botany (Impact Factor: 1.4). 01/2011; 57(12):1332-1334. DOI: 10.1139/b79-163


A simple, rapid method requiring few manipulations for the extraction of chlorophylls from fragmented leaf tissue of angiosperms and gymnosperms is compared with the widely used acetone method. Unlike the acetone method where grinding and subsequent centrifugation are essential, this method makes use of incubation at 65 °C of leaf tissue immersed in dimethyl sulphoxide. The new method was found to be as efficient as acetone for chlorophyll extraction and superior in terms of chlorophyll stability.

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    • "The green leaves were taken for chlorophyll estimation according to the method of Hiscox and Israelstam ( 1979 ) . One hundred mg of tomato leaf pieces were kept in a vial containing 7ml of a chemical dimethyl sulfoxide ( DMSO ) and the chlorophyll content was extracted into the fluid by incubating for 60min . "
    12/2015; 1(1):1068523. DOI:10.1080/23311932.2015.1068523
    • "CRI stage. Photosynthetic pigments like chlorophyll a and b were estimated according to the method of Hiscox and Israel and Tam (1979 using dimethyl sulphoxide (DMSO) chemical under laboratory conditions. Chlorophyll concentration index (CCI) was recorded using Apogee's CCM-200 instrument. "
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    ABSTRACT: Abstract A field experiment was conducted for three cultivars of wheat i.e. PBW 343, PBW550 and DBW17 with five nitrogen levels i.e. 0, 75, 125, 175 and 225 kg N/ha in split plot design. Growth parameters like LAI, chlorophyll content, nitrogen, chlorophyll concentration index (CCI) and plant height were recorded. Spectral indices such as Normalized difference Vegetation Index (NDVI), Ratio Vegetation Index (RVI), Moisture Stress Index (MSI), Green Index (GI), Leaf Chlorophyll Index (LCI), Plant Senescence Reflectance Index (PSRI), were computed using the multiband spectral data. Among the different phenological phases of crop growth, best correlations were observed between different growth parameters and vegetation indices at booting stage of wheat. The spectral indices selected for the study recorded at booting stage were correlated better with all the crop parameters having ‘R2’ values varied between 0.68 and 0.83. All the spectral indices were having good correlation (R2) ranging between 0.58 and 0.82 for grain yield and 0.63–0.80 for 1000 grain weight, But best correlation were noticed between NDVI and grain yield having R2 value 0.78, NDVI and 1000 grain wt having R2 value 0.80, LCI and grain yield having R2 value 0.82, LCI and 1000 grain weight having R2 value 0.79. The LCI and NDVI were having best correlation with grain yield and 1000 grain wt. It was concluded that amongst the various indices selected, Leaf color index (LCI) was the only index which was correlated better, with all the plant growth parameters and yield at booting stage of the wheat crop.
    Journal of the Indian Society of Remote Sensing 09/2015; 43(3):551-558. · 0.76 Impact Factor
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    • "Foliar application was performed using a 3-L pre-compression sprayer, made of high molecular weight polyethylene, with piston-type pump and a nozzle of 34 mm of diameter. Part of the leaf biomass was collected 5 days after the beginning of flowering, for the determination of the following variables: contents of photosynthetic pigments, chlorophylls 'a' (Chl a), 'b' (Chl b), total chlorophyll (Chl t) and carotenoids (Car), expressed in µmol m -2 (Arnon, 1949; Hiscox & Israelstam, 1979), besides cell extrusion (Ext) and relative water content in the leaves (RWC) (Brito et al., 2011). "
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    ABSTRACT: The castor bean crop plays an important social, environmental and economic role, especially due to possibility of its use as feedstock for the generation of clean energy. Thus, this study aimed to evaluate the contents of photosynthetic pigments, cell membrane extrusion and the relative water content in the leaves of the castor bean cultivar ‘BRS Energia’ under silicon and salinity levels. The experiment was carried out at Embrapa Cotton, in a completely randomized design, in a 4 x 4 factorial scheme, and the treatments consisted of four silicon levels (0, 100, 200 and 300 mg L-1) and four water salinity levels of (ECw 0, 2, 4 and 6 dS m-1), with three replicates. The gradual increase in salinity levels reduced the concentration of photosynthetic pigments, relative water content in the leaves and increased cell membrane extrusion. The increase in the concentration of silicon reduced the harmful effects of salts on the analysed variables in the castor bean ‘BRS Energia’.
    Revista Brasileira de Engenharia Agrícola e Ambiental 06/2015; 19(9):841-848. DOI:10.1590/1807-1929/agriambi.v19n9p841-848 · 0.55 Impact Factor
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