Article

Structure elucidation and complete NMR spectral assignment of two triterpenoid saponins from Radix Hedysari

State Key Laboratory of Natural and Biomimetic Drugs, Peking University and Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China.
Fitoterapia (Impact Factor: 2.22). 03/2009; 80(2):127-9. DOI: 10.1016/j.fitote.2008.12.001
Source: PubMed

ABSTRACT A new oleanane-type triterpenoid saponin with a carbonyl group at C-15, named polybosaponin A (1), together with a known triterpenoid saponin, dehydrosoyasaponin I (2), were isolated from Radix Hedysari. On the basis of one- and two-dimensional NMR and high resolution electrospray ionization mass spectrometry, the structure of 1 were elucidated and the (1)H and (13)C NMR spectral signals were assigned totally. In addition, the complete assignment of (1)H NMR signals and revision of incorrect assignment of (13)C NMR signals for 2 were also achieved based on one- and two-dimensional NMR spectrometry.

0 Followers
 · 
121 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: AimTo study the chemical constituents from the rhizome of Hedysarum polybotrys.
    Chinese Journal of Natural Medicines 01/2009; 7(5):351-353. DOI:10.3724/SP.J.1009.2009.00351
  • [Show abstract] [Hide abstract]
    ABSTRACT: Radix Astragali and Radix Hedysari, two famous traditional Chinese medicines (TCM), were considered to possess the same efficacy in TCM traditionally. However, modern pharmacological and phytochemical investigations showed that they were different in efficacy and chemical constituents to some degree. In this study, the principal constituents of the two drugs, isoflavonoids and saponins, were comparatively analysed using HPLC-UV and HPLC-ELSD methods. The results showed that there were some common compounds, especially flavonoids, in both drugs, while the differences in the types and contents of their chemical constituents, especially in saponins, were also obvious. The similarities and differences of the chemicals may be responsible, at least partially, for the similarities and differences in their efficacies or bioactivities.
    Natural product research 10/2011; 26(20):1935-8. DOI:10.1080/14786419.2011.619188 · 1.23 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: To investigate the effect of total flavonoids of Hedysarum polybotry on the proliferation, cell cycle, and expressions of p21(Ras) and proliferating cell nuclear antigen (PCNA) gene in erythroleukemia cell line K562. The effect of total flavonoids of Hedysarum polybotry on K562 cell line survival was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) reduction assay. The time- and dose-dependent manner was also observed. The cell cycle and apoptosis were analyzed with flow cytometry (FCM). The immunocytochemistry method was applied to quantitatively analyze the effects of flavonoids of Hedysarum polybotry on changes p21(Ras) and PCNA gene expressions. Flavonoids of Hedysarum polybotry (20-100 μg/mL) significantly inhibited the proliferation of K562 cells in a time- and dose-dependent manner. After K562 cells were cultured for 48 h, total flavonoids of Hedysarum polybotry had no significant effect on the apoptosis of K562 cells but showed significantly inhibition (P<0.01), indicating that total flavonoids of Hedysarum polybotry could induce K562 cells arrested at G(0)/G(1) and G(2)/M phases. Compared with the control group, p21(Ras) and PCNA gene expressions were decreased significantly in K562 cells treated with total flavonoids of Hedysarum polybotry (40 and 80 μg/mL, respectively) for 48 h. The inhibitory effect on proliferation of K562 cells was observed in the groups treated with flavonoids of Hedysarum polybotry, which might be related to cells arresting.
    Chinese Journal of Integrative Medicine 01/2012; 18(5):385-90. DOI:10.1007/s11655-011-0952-3 · 1.40 Impact Factor