Article

[Study on the roles of voltaged potassium channels in acute hypoxic pulmonary vasoconstriction].

Department of Anesthesiology, People's Hospital of Guangdong Province, Guangzhou 510080, China. E-mail: .
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 01/2009; 28(12):2200-1. pp.2200-1
Source: PubMed

ABSTRACT To investigate the role of voltage-gated potassium channels in the acute hypoxic pulmonary vasoconstriction.
Thirty Wistar rats were divided into two groups, namely the normoxic group and hypoxic group. The single smooth muscle cell was obtained from the pulmonary artery of Wistar rats with acute enzymatic digestion method. The conventional whole-cell patch clamp technique was used to record the resting membrane potential(Em) and the potassium currents of voltage-gated potassium channel (IKv) in rat pulmonary arterial smooth muscle cells(PASMC). Intracellular application of Kv1.2, Kv1.3, Kv1.5, and Kv2.1 antibodiesè1:125éwas conducted through the whole-cell patch clamp system.
Em of PASMC was depolarized in hypoxia compared with that of control cells. The mixture of Kv1.2, Kv1.3, Kv1.5, and Kv2.1 antibodiesè1:125é depolarized Em and inhibited Ikv in PASMC from normoxic rat,whereas the mixture of Kv1.2, Kv1.3, Kv1.5, Kv2.1 antibodiesè1:125éhad no effects on Ikv and Em in hypoxic rats.
Kv1.2, Kv1.3, Kv1.5, Kv2.1 might be oxygen sensitive potassium channels which mediated acute hypoxic pulmonary vasoconstriction.

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Keywords

acute enzymatic digestion method
 
acute hypoxic pulmonary vasoconstriction
 
control cells
 
conventional whole-cell patch clamp technique
 
Em
 
hypoxia
 
hypoxic group
 
hypoxic rats
 
IKv
 
inhibited Ikv
 
Intracellular application
 
mediated acute hypoxic pulmonary vasoconstriction
 
rat pulmonary arterial smooth muscle cells(PASMC)
 
resting membrane potential(Em)
 
single smooth muscle cell
 
voltage-gated potassium channel
 
voltage-gated potassium channels
 
whole-cell patch clamp system